Department of Ecosystem and Public Health, Faculty of Veterinary Medicine, University of Calgary, Calgary, AB, Canada.
Animal Policy and Epidemiology Section, Animal Health Branch, Animal Health and Assurance Division, Alberta Agriculture and Forestry, Edmonton, AB, Canada.
Front Cell Infect Microbiol. 2021 Mar 12;11:622195. doi: 10.3389/fcimb.2021.622195. eCollection 2021.
Horizontal gene transfer is an important mechanism which facilitates bacterial populations in overcoming antimicrobial treatment. In this study, a total of 120 and 62 subsp. isolates were isolated from broiler chicken farms in Alberta. Fourteen serovars were identified among isolates. Thirty one percent of isolates (37/120) were multiclass drug resistant (resistant to ≥ 3 drug classes), while only about 16% of isolates (10/62) were multiclass drug resistant. Among those, eight isolates had an AmpC-type phenotype, and one isolate had an extended-spectrum beta-lactamase (ESBL)-type beta-lactamase phenotype. We identified both AmpC-type () and ESBL-type () genes in both and isolates. Plasmids from eight of nine and isolates were transferred to recipient strain J53 through conjugation. Transferable plasmids in the eight and isolates were also transferred into a lab-made sodium azide-resistant recipient through conjugation. The class 1 integrase gene, , was detected on plasmids from two isolates. Further investigation of class 1 integron cassette regions revealed the presence of an gene encoding streptomycin 3''-adenylyltransferase, an / gene encoding aminoglycoside 3''-O-adenyltransferase, and a putative adenylyltransferase gene. This study provides some insight into potential horizontal gene transfer events of antimicrobial resistance genes between and in broiler chicken production.
水平基因转移是促进细菌种群克服抗菌治疗的重要机制。在这项研究中，从艾伯塔省的肉鸡养殖场共分离出 120 株和 62 株亚种。在分离株中鉴定出 14 种血清型。31%的 分离株（37/120）为多药耐药（对≥3 种药物类别耐药），而只有约 16%的 分离株（10/62）为多药耐药。其中，8 株分离株具有 AmpC 型表型，1 株分离株具有超广谱β-内酰胺酶（ESBL）型β-内酰胺酶表型。我们在 和 分离株中均鉴定出 AmpC 型（）和 ESBL 型（）基因。来自 8 株 和 分离株的质粒通过共轭转移到受体菌株 J53 中。在 8 株 和 分离株中的可转移质粒也通过共轭转移到实验室制备的耐叠氮化钠的 受体中。在两个 分离株的质粒上检测到类 1 整合酶基因 。对类 1 整合子盒区的进一步研究表明，存在一个编码链霉素 3''-腺苷酰转移酶的 基因、一个编码氨基糖苷 3''-O-腺苷酰转移酶的 / 基因和一个推定的腺苷酰转移酶基因。本研究为肉鸡生产中 和 之间抗菌药物耐药基因的潜在水平基因转移事件提供了一些见解。
