Hassan Walid Hamdy, Salam Hala Sayed Hassan, Hassan Wafaa Mohamed, Shany Salama Abohamra Sayed, Osman Ghada Sayed Ibrahim
Department of Bacteriology, Mycology and Immunology, Faculty of Veterinary Medicine, Beni-Suef University, Beni-Suef, Egypt.
Microbiology-Reference Laboratory of Quality Control on Poultry Production, Animal Health Research Institute AHRI, Giza Governorate, Egypt.
J Adv Vet Anim Res. 2022 Jun 26;9(2):191-202. doi: 10.5455/javar.2022.i584. eCollection 2022 Jun.
This study aimed to prove the effects of isolates isolated from diseased broilers to form biofilms, describe their antimicrobial sensetivity, and determine the effect of allicin and cinnamon essential oils on the expression of some genes (H, 1, and S) through quantitative polymerase chain reaction (q-PCR).
140 samples were obtained from diseased broilers in Beni-Suef Governorate, Egypt. These samples were examined by conventional bacteriology methods to detect the causative agent. The antimicrobial susceptibility of the isolated bacteria was assessed using the disc diffusion method, The ability of yeast extract-casamino acids Congo Red Agar to generate phenotypic biofilms was next tested. The presence of resistance and virulence genes in some multidrug resistant isolates was genotypically investigated. The antibacterial effects of allicin and cinnamon oil were evaluated against the growth of multidrug-resistant . Finally, q-PCR was utilized to assess changes in some genes' expression.
was isolated from 61 samples (43.6%). An antimicrobial susceptibility test revealed that multidrug-resistance (MDR) (could resist more than three antimicrobial classes) prevalence was 100%. 40.8% of isolates phenotypically produce biofilms. The detection of resistance and virulence genes by PCR showed that all tested isolates carry B, H, 1, S, and S genes, while only 40% harbor genes. q-PCR showed that after treatment with allicin and cinnamon oils, gene expression went down.
This investigation highlights that showed resistance against most of the tested antimicrobials; all isolates were MDR. The study showed wide dissemination of virulence and resistance genes among . Allicin and cinnamon oils have antimicrobial activities and could be used as alternatives to synthetic antimicrobial agents.
本研究旨在证实从患病肉鸡中分离出的菌株形成生物膜的作用，描述其抗菌敏感性，并通过定量聚合酶链反应（q-PCR）确定大蒜素和肉桂精油对某些基因（H、1和S）表达的影响。
从埃及贝尼苏韦夫省的患病肉鸡中获取140份样本。通过常规细菌学方法检查这些样本以检测病原体。使用纸片扩散法评估分离细菌的抗菌敏感性，接下来测试酵母提取物 - 酪蛋白氨基酸刚果红琼脂产生表型生物膜的能力。对一些多重耐药菌株进行基因型研究，以检测耐药性和毒力基因的存在。评估大蒜素和肉桂油对多重耐药菌生长的抗菌作用。最后，利用q-PCR评估某些基因表达的变化。
从61份样本（43.6%）中分离出[具体细菌名称未给出]。抗菌敏感性测试显示多重耐药（MDR）（可抵抗超过三类抗菌药物）的发生率为100%。40.8%的分离株在表型上产生生物膜。通过PCR检测耐药性和毒力基因表明，所有测试分离株均携带B、H、1、S和S基因，而只有40%携带[具体基因名称未给出]基因。q-PCR显示，用大蒜素和肉桂油处理后，基因表达下降。
本研究突出表明[具体细菌名称未给出]对大多数测试抗菌药物具有抗性；所有分离株均为多重耐药。该研究表明毒力和耐药基因在[具体细菌名称未给出]中广泛传播。大蒜素和肉桂油具有抗菌活性，可作为合成抗菌剂的替代品。
