一种遗传模型揭示了在小鼠体内经历稳态的新生CD4辅助性T细胞的生物学特征。
A Genetic Model Reveals Biological Features of Neonatal CD4 Helper Cells Undergone Homeostasis in Mice.
作者信息
Lei Lei, Zhang Xingzhe, Yang Xiaofeng, Su Yanhong, Liu Haiyan, Yang Hang, Wang Jinli, Zou Yujing, Wang Xin, Jiao Anjun, Zhang Cangang, Zheng Huiqiang, Zhang Jiahui, Zhang Dan, Shi Lin, Zhou Xiaobo, Sun Chenming, Zhang Baojun
机构信息
Department of Pathogenic Microbiology and Immunology, School of Basic Medical Sciences, Xi'an Jiaotong University, Xi'an, China.
Institute of Infection and Immunity, Translational Medicine Institute, Xi'an Jiaotong University Health Science Center, Xi'an, China.
出版信息
Front Cell Dev Biol. 2021 Mar 11;9:659744. doi: 10.3389/fcell.2021.659744. eCollection 2021.
CD4 T cells are essential for regulating effective immune response to pathogens and immune balance. Recent studies have demonstrated the unique features of T cells in neonate mice, such as more sensitive to antigen response and preference toward T helper 2 (Th2) response and regulatory T cells (Tregs) differentiation. However, the biological characteristics of neonatal age-derived CD4 T cells following homeostasis remain unclear. Here we utilized a lineage tracing model of δ to mark neonatal- and adult-derived CD4 T cells followed by a combination analysis of activation, proliferation, survival, and differentiation. Our results showed that neonatal CD4 T cells had higher capacity of activation, proliferation, apoptosis, and differentiation toward Th2 and T helper 17 (Th17) lineages, accompanied by a reduced potential for T helper 1 (Th1), T helper 9 (Th9), and Treg lineages. In contrast, tracked neonatal CD4 T cells exhibited similar characters of above-mentioned of tracked adult cells in adult mice. Therefore, our data support a natural requirement for CD4 T cells to acquire fully-equipped functional potentials of adult cells.
CD4 T细胞对于调节针对病原体的有效免疫反应和免疫平衡至关重要。最近的研究已经证明了新生小鼠T细胞的独特特征，例如对抗原反应更敏感，倾向于T辅助2（Th2）反应和调节性T细胞（Tregs）分化。然而，稳态后新生期来源的CD4 T细胞的生物学特性仍不清楚。在这里，我们利用δ谱系追踪模型来标记新生和成年来源的CD4 T细胞，随后对激活、增殖、存活和分化进行综合分析。我们的结果表明，新生CD4 T细胞具有更高的激活、增殖、凋亡能力，以及向Th2和T辅助17（Th17）谱系分化的能力，同时向T辅助1（Th1）、T辅助9（Th9）和Treg谱系分化的潜力降低。相比之下，在成年小鼠中，追踪到的新生CD4 T细胞表现出与追踪到的成年细胞上述类似的特征。因此，我们的数据支持CD4 T细胞自然需要获得成年细胞完全具备的功能潜力。
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