State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, 430070 Hubei, China.
National Center for International Research on Animal Genetics, Breeding and Reproduction, Huazhong Agricultural University, Wuhan, 430070, China.
Biomed Res Int. 2021 Mar 12;2021:6667715. doi: 10.1155/2021/6667715. eCollection 2021.
The gut microbiota represents a source of genetic and metabolic diversity of a complex polymicrobial ecosystem within its host. To investigate age-based variations of the gut microbiota among Shennongjia golden snub-nosed monkeys (), we characterized the microbial species in fecal samples from 18 Shennongjia golden snub-nosed monkeys evenly pooled into 3 aged groups (Group 1, 1-3 years; Group 2, 5-8 years; Group 3, above 12 years) in Shennongjia, Hubei Province, China. Genomic DNA was extracted from fecal samples, and the 16S rRNA gene V4 region was sequenced using the Illumina high-throughput MiSeq platform PE250. A total of 28 microbial phyla were identified in the gut microbiome of these monkeys with the ten most abundant phyla (i.e., Firmicutes, Bacteroidetes, Verrucomicrobia, Spirochaetes, Tenericutes, Proteobacteria, Planctomycetes, Fibrobacteres, Cyanobacteria, and Euryarchaeota). A total of 1,469 (of 16 phyla and 166 genera), 1,381 (of 16 phyla and 157 genera), and 1,931 (of 19 phyla and 190 genera) operational taxonomic units (OTUs) were revealed in Groups 1, 2, and 3, respectively, with Group 3 containing the most diverse groups of OTUs as revealed by the species relative abundance clustering analysis. These results suggest that the gut microbiota in these monkeys maintain a dynamic status, starting from the early developmental stages of life with the species relative abundance increasing with age. This is the first study to comprehensively characterize the gut microbiota and provide valuable information for monitoring the health and nutritional needs of this endangered primate at different ages.
肠道微生物群代表了宿主复杂多微生物生态系统的遗传和代谢多样性来源。为了研究神农架金丝猴肠道微生物群随年龄的变化，我们对来自湖北省神农架的 18 只金丝猴的粪便样本进行了特征分析，这些猴子被平均分为 3 个年龄组（第 1 组，1-3 岁；第 2 组，5-8 岁；第 3 组，12 岁以上）。从粪便样本中提取基因组 DNA，使用 Illumina 高通量 MiSeq 平台 PE250 对 16S rRNA 基因 V4 区进行测序。在这些猴子的肠道微生物组中共鉴定出 28 个微生物门，其中最丰富的 10 个门（厚壁菌门、拟杆菌门、疣微菌门、螺旋体门、无壁菌门、变形菌门、浮霉菌门、纤维杆菌门、蓝藻门和古菌门）。第 1 组、第 2 组和第 3 组分别有 1,469 个（16 个门和 166 个属）、1,381 个（16 个门和 157 个属）和 1,931 个（19 个门和 190 个属）OTUs，物种相对丰度聚类分析显示第 3 组的 OTUs 多样性最多。这些结果表明，这些猴子的肠道微生物群保持着动态状态，从生命的早期发育阶段开始，随着年龄的增长，物种相对丰度增加。这是首次全面描述肠道微生物群的研究，为监测不同年龄阶段这种濒危灵长类动物的健康和营养需求提供了有价值的信息。
