Hong Yanyun, Duo Hairui, Hong Juyun, Yang Jinyuan, Liu Shiming, Yu Lianghui, Yi Tuyong
College of Plant Protection of Hunan Agricultural University Changsha China.
Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Plant Pests Changsha China.
Ecol Evol. 2017 May 15;7(12):4456-4464. doi: 10.1002/ece3.3011. eCollection 2017 Jun.
Shennongjia (SNJ ) is the smallest geographical population of . . The phylogenetic relationships among its genera and species and the biogeographic processes leading to their current distribution are largely unclear. To address these issues, we resequenced and obtained a new, complete mitochondrial genome of SNJ by next-generation sequencing and standard Sanger sequencing. We analyzed the gene composition, constructed a phylogenetic tree, inferred the divergence ages based on complete mitochondrial genome sequences, and analyzed the genetic divergence of 13 functional mtDNA genes. The phylogenetic tree and divergence ages showed that (the Tonkin snub-nosed monkey) was the first to diverge from the genus ca. 2.47 million years ago (Ma) and formed sister groups, and the second divergence from the genus occurred ca. 1.90 Ma. and diverged from the genus third, ca. 1.57 Ma. SNJ was the last to diverge within species in 0.08 Ma, and the most recent common ancestor of is 0.10 Ma. The analyses on gene composition showed SNJ was the newest geographic population of . The work will help to develop a more accurate protection policy for SNJ and facilitate further research on selection and adaptation of .
神农架（SNJ）是……最小的地理种群。其属种间的系统发育关系以及导致它们当前分布的生物地理过程在很大程度上尚不清楚。为解决这些问题，我们通过新一代测序和标准桑格测序对SNJ进行了重测序，并获得了一个新的、完整的线粒体基因组。我们分析了基因组成，构建了系统发育树，基于完整的线粒体基因组序列推断了分歧时间，并分析了13个功能性线粒体DNA基因的遗传分歧。系统发育树和分歧时间表明，（越南金丝猴）约在247万年前（Ma）首先从仰鼻猴属分化出来，与（滇金丝猴）形成姐妹群，第二次从仰鼻猴属分化发生在约190万年前。（黔金丝猴）和（川金丝猴）第三次从仰鼻猴属分化出来，约在157万年前。神农架金丝猴在0.08万年前是仰鼻猴属物种中最后分化出来的，其最近共同祖先为0.10万年前。对基因组成的分析表明，神农架金丝猴是仰鼻猴属最新的地理种群。这项工作将有助于为神农架金丝猴制定更准确的保护政策，并促进对仰鼻猴属选择和适应的进一步研究。
