Rojas Connie A, Entrolezo Zhandra, Jarett Jessica K, Jospin Guillaume, Kingsbury Dawn D, Martin Alex, Eisen Jonathan A, Ganz Holly H
Genome Center, University of California, Davis, CA 95616, USA.
Department of Evolution and Ecology, University of California, Davis, CA 95616, USA.
Vet Sci. 2023 Sep 6;10(9):561. doi: 10.3390/vetsci10090561.
There is growing interest in the application of fecal microbiota transplants (FMTs) in small animal medicine, but there are few published studies that have tested their effects in the domestic cat (). Here we use 16S rRNA gene sequencing to examine fecal microbiome changes in 46 domestic cats with chronic digestive issues that received FMTs using lyophilized stool that was delivered in oral capsules. Fecal samples were collected from FMT recipients before and two weeks after the end of the full course of 50 capsules, as well as from their stool donors (N = 10), and other healthy cats (N = 113). The fecal microbiomes of FMT recipients varied with host clinical signs and dry kibble consumption, and shifts in the relative abundances of , , , and were observed after FMT. Overall, donors shared 13% of their bacterial amplicon sequence variants (ASVs) with FMT recipients and the most commonly shared ASVs were classified as 9, , , and . Lastly, the fecal microbiomes of cats with diarrhea became more similar to the microbiomes of age-matched and diet-matched healthy cats compared to cats with constipation. Overall, our results suggest that microbiome responses to FMT may be modulated by the FMT recipient's initial presenting clinical signs, diet, and their donor's microbiome.
粪便微生物群移植（FMT）在小动物医学中的应用越来越受到关注，但很少有已发表的研究测试过其对家猫的效果。在这里，我们使用16S rRNA基因测序来检测46只患有慢性消化问题的家猫在接受口服胶囊冻干粪便进行FMT后的粪便微生物组变化。在50粒胶囊的整个疗程结束前和结束后两周，从FMT接受者以及它们的粪便供体（N = 10）和其他健康猫（N = 113）中收集粪便样本。FMT接受者的粪便微生物组因宿主临床症状和干粮摄入量而异，FMT后观察到、、、和的相对丰度发生了变化。总体而言，供体与FMT接受者共享了13%的细菌扩增子序列变体（ASV），最常见的共享ASV被分类为9、、、和。最后，与便秘猫相比，腹泻猫的粪便微生物组与年龄和饮食匹配的健康猫的微生物组更相似。总体而言，我们的结果表明，微生物组对FMT的反应可能受到FMT接受者最初呈现的临床症状、饮食及其供体微生物组的调节。
