Valencia C Sandra Y, Isaza M Carlos A, Henao B Julieta, Beltrán A Leonardo, Loango Nelsy, Landázuri Patricia
Faculty of Health Sciences, Program of Nutrition, Universidad Libre, Colombia.
Faculty of Medicine, Fundación Universitaria Autónoma de las Américas, Colombia.
Biochem Biophys Rep. 2021 Mar 18;26:100971. doi: 10.1016/j.bbrep.2021.100971. eCollection 2021 Jul.
Controversy exists regarding the role of the subfractions of high-density lipoproteins (HDL and HDL) in cardiovascular disease. The functionality of these particles, and their protective role, is due in part to the paraoxonase 1 (PON1) presence in them. The polymorphisms rs662 (Q192R, A/G), rs854560 (L55 M, T/A), and rs705379 (C-108T) of the PON1 gene have been related to enzyme activity and, with the anti-oxidative capacity of the HDL. The objective was to determine the arylesterase PON1 activity in HDL and HDL and its relationship with the polymorphisms mentioned, in a young population.
The polymorphisms were determined through mini-sequencing (SnaPshot). The HDL subpopulations were separated via ionic precipitation, cholesterol was measured with enzymatic methods, and PON1 activity was measured through spectrophotometry.
The results show that the PON1 polymorphisms do not influence the cholesterol in the HDL. A variation between 40.02 and 43.9 mg/dL was in all the polymorphisms without significant differences. Additionally, PON1 activity in the HDL subfractions was greater (62.83 ± 20 kU/L) than with HDL (35.8 ± 20.8 kU/L) in the whole population and in all the polymorphisms (p < 0.001), and it was independent of the polymorphism and differential arylesterase activity in the Q192R polymorphism (QQ > QR > RR). Thus, 115.90 ± 30.7, 88.78 ± 21.3, 65.29 ± 10.2, respectively, for total HDL, with identical behavior for HDL and HDL.
PON1 polymorphisms do not influence the HDL, and the PON activity is greater in the HDL than in the HDL, independent of the polymorphism, but it is necessary to delve into the functionality of these findings in different populations.
关于高密度脂蛋白（HDL）亚组分在心血管疾病中的作用存在争议。这些颗粒的功能及其保护作用部分归因于其中存在的对氧磷酶1（PON1）。PON1基因的多态性rs662（Q192R，A/G）、rs854560（L55M，T/A）和rs705379（C-108T）与酶活性以及HDL的抗氧化能力有关。目的是确定年轻人群中HDL和HDL中芳基酯酶PON1的活性及其与上述多态性的关系。
通过微测序（SnaPshot）确定多态性。通过离子沉淀分离HDL亚群，用酶法测量胆固醇，用分光光度法测量PON1活性。
结果表明，PON1多态性不影响HDL中的胆固醇。所有多态性中的胆固醇含量在40.02至43.9mg/dL之间变化，无显著差异。此外，在整个人群和所有多态性中，HDL亚组分中的PON1活性（62.83±20kU/L）高于HDL（35.8±20.8kU/L）（p<0.001），并且它独立于Q192R多态性中的多态性和差异芳基酯酶活性（QQ>QR>RR）。因此，总HDL分别为115.90±30.7、88.78±21.3、65.29±10.2，HDL和HDL具有相同的行为。
PON1多态性不影响HDL，并且HDL中的PON活性高于HDL，与多态性无关，但有必要深入研究这些发现在不同人群中的功能。
