Liu Luxian, Zhang Yonghua, Li Pan
Key Laboratory of Plant Stress Biology, School of Life Sciences, Henan University, Kaifeng, 475000, China.
College of Life and Environmental Sciences, Wenzhou University, Wenzhou, 325035, China.
Plant Divers. 2020 Oct 5;43(1):43-53. doi: 10.1016/j.pld.2020.09.005. eCollection 2021 Feb.
is a Cannabaceae genus of 60-70 species of trees, or rarely shrubs, commonly known as hackberries. This woody genus consists of very valuable forest plants that provide important wildlife habitat for birds and mammals. Although previous studies have identified its phylogenetic position, interspecific relationships within remain unclear. In this study, we generated genome skimming data from five species to analyze phylogenetic relationships within the genus and develop genome resources. The plastomes of ranged in length from 158,989 bp to 159,082 bp, with a typical angiosperm quadripartite structure, and encoded a total of 132 genes with 20 duplicated in the IRs. Comparative analyses showed that plastome content and structure were relatively conserved. Whole plastomes showed no signs of gene loss, translocations, inversions, or genome rearrangement. Six plastid hotspot regions (-, -, , -, and ), 4097 polymorphic nuclear SSRs, as well as 62 low or single-copy gene fragments were identified within . Moreover, the phylogenetic relationships based on the complete plastome sequences strongly endorse the placement of as sister to the ((((, ), ), ), ) clade. These findings and the genetic resources developed here will be conducive to further studies on the genus involving phylogeny, population genetics, and conservation biology.
是大麻科的一个属，包含60 - 70种乔木，极少为灌木，通常被称为朴树。这个木本属由非常有价值的森林植物组成，为鸟类和哺乳动物提供重要的野生动物栖息地。尽管先前的研究已经确定了它的系统发育位置，但该属内的种间关系仍不清楚。在本研究中，我们从五个物种中生成了基因组浅层测序数据，以分析该属内的系统发育关系并开发基因组资源。朴树属的质体基因组长度在158,989 bp至159,082 bp之间，具有典型的被子植物四分体结构，共编码132个基因，其中20个在反向重复序列（IRs）中重复。比较分析表明，质体基因组的内容和结构相对保守。整个质体基因组没有基因丢失、易位、倒位或基因组重排的迹象。在朴树属内鉴定出六个质体热点区域（ - ， - ， ， - ， 和 ）、4097个多态性核简单序列重复（SSRs）以及62个低拷贝或单拷贝基因片段。此外，基于完整质体基因组序列的系统发育关系有力地支持朴树属作为（((( ， )， )， )， )分支的姐妹群的位置。这些发现以及在此开发的遗传资源将有助于对朴树属进行进一步的系统发育、群体遗传学和保护生物学研究。
