Wang Mingcheng, Lin Haifeng, Lin Hongqiang, Du Panyue, Zhang Shuqiao
Institute for Advanced Study, Chengdu University, No. 2025 Chengluo Road, Chengdu 610106, China.
Engineering Research Center of Sichuan-Tibet Traditional Medicinal Plant, Chengdu 610106, China.
Genes (Basel). 2024 Dec 26;16(1):16. doi: 10.3390/genes16010016.
BACKGROUND/OBJECTIVES: Modern sequencing technologies have transformed the identification of medicinal plant species and varieties, overcoming the limitations of traditional morphological and chemical approaches. This review explores the key DNA-based techniques, including molecular markers, DNA barcoding, and high-throughput sequencing, and their contributions to enhancing the accuracy and reliability of plant identification. Additionally, the integration of multi-omics approaches is examined to provide a comprehensive understanding of medicinal plant identity.
The literature search for this review was conducted across databases such as Google Scholar, Web of Science, and PubMed, using keywords related to plant taxonomy, genomics, and biotechnology. Inclusion criteria focused on peer-reviewed studies closely related to plant identification methods and techniques that contribute significantly to the field.
The review highlights that while sequencing technologies offer substantial improvements, challenges such as high costs, technical expertise, and the lack of standardized protocols remain barriers to widespread adoption. Potential solutions, including AI-driven data analysis and portable sequencers, are discussed.
This review provides a comprehensive overview of molecular techniques, their transformative impact, and future perspectives for more accurate and efficient medicinal plant identification.
背景/目标：现代测序技术改变了药用植物物种和品种的鉴定方式，克服了传统形态学和化学方法的局限性。本综述探讨了基于DNA的关键技术，包括分子标记、DNA条形码和高通量测序，以及它们对提高植物鉴定准确性和可靠性的贡献。此外，还研究了多组学方法的整合，以全面了解药用植物特性。
本综述的文献检索在谷歌学术、科学网和PubMed等数据库中进行，使用与植物分类学、基因组学和生物技术相关的关键词。纳入标准侧重于与植物鉴定方法和技术密切相关且对该领域有重大贡献的同行评审研究。
该综述强调，虽然测序技术有显著改进，但高成本、技术专业知识以及缺乏标准化方案等挑战仍然是广泛应用的障碍。讨论了包括人工智能驱动的数据分析和便携式测序仪在内的潜在解决方案。
本综述全面概述了分子技术、它们的变革性影响以及更准确、高效地鉴定药用植物的未来前景。
