Zhang Huanlei, Jin Jianjun, Moore Michael J, Yi Tingshuang, Li Dezhu
Germplasm Bank of Wild Species, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China.
Kunming College of Life Sciences, University of Chinese Academy of Sciences, Kunming 650201, China.
Plant Divers. 2018 Apr 23;40(3):127-137. doi: 10.1016/j.pld.2018.04.003. eCollection 2018 Jun.
Cannabaceae is an economically important family that includes ten genera and ca. 117 accepted species. To explore the structure and size variation of their plastomes, we sequenced ten plastomes representing all ten genera of Cannabaceae. Each plastome possessed the typical angiosperm quadripartite structure and contained a total of 128 genes. The Inverted Repeat (IR) regions in five plastomes had experienced small expansions (330-983 bp) into the Large Single-Copy (LSC) region. The plastome of has experienced a 942-bp IR contraction and lost and in its IRs. The substitution rates of and decreased after they shifted from the LSC to IR. A 270-bp inversion was detected in the plastome, which might have been mediated by 18-bp inverted repeats. Repeat sequences, simple sequence repeats, and nucleotide substitution rates varied among these plastomes. Molecular markers with more than 13% variable sites and 5% parsimony-informative sites were identified, which may be useful for further phylogenetic analysis and species identification. Our results show strong support for a sister relationship between and (BS = 100). , -, -, and - formed a strongly supported clade, and their relationships were well resolved with strong support (BS = 100). The availability of these ten plastomes provides valuable genetic information for accurately identifying species, clarifying taxonomy and reconstructing the intergeneric phylogeny of Cannabaceae.
大麻科是一个具有重要经济意义的科，包括10个属和约117个公认的物种。为了探究其质体基因组的结构和大小变异，我们对代表大麻科所有10个属的10个质体基因组进行了测序。每个质体基因组都具有典型的被子植物四分体结构，共包含128个基因。五个质体基因组中的反向重复（IR）区域向大单拷贝（LSC）区域发生了小的扩张（330 - 983 bp）。[此处原文缺失具体属名]的质体基因组经历了942 bp的IR收缩，并在其IR区域丢失了[此处原文缺失具体基因名]和[此处原文缺失具体基因名]。[此处原文缺失具体属名]和[此处原文缺失具体属名]从LSC转移到IR后，其替换率降低。在[此处原文缺失具体属名]的质体基因组中检测到一个270 bp的倒位，这可能是由18 bp的反向重复介导的。这些质体基因组中的重复序列、简单序列重复和核苷酸替换率各不相同。鉴定出了可变位点超过13%且简约信息位点超过5%的分子标记，这可能有助于进一步的系统发育分析和物种鉴定。我们的结果有力支持了[此处原文缺失具体属名]和[此处原文缺失具体属名]之间的姐妹关系（BS = 100）。[此处原文缺失具体属名]、[此处原文缺失具体属名]、[此处原文缺失具体属名]和[此处原文缺失具体属名]形成了一个得到有力支持的分支，它们之间的关系得到了很好的解析且支持度很高（BS = 100）。这10个质体基因组的可得性为准确鉴定物种、阐明分类学以及重建大麻科的属间系统发育提供了有价值的遗传信息。
