Basak Soumyadeep, Kumar Vinay, Packirisamy Gopinath
Department of Biotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
Nanobiotechnology Laboratory, Centre for Nanotechnology, Indian Institute of Technology Roorkee, Roorkee 247667, Uttarakhand, India.
ACS Omega. 2021 Mar 12;6(11):7605-7614. doi: 10.1021/acsomega.0c06135. eCollection 2021 Mar 23.
Hanging cell culture inserts are most widely used cell culture devices, which provide a freestanding multichamber setup for various co-culture and triculture systems. Apart from being costly, the commercial inserts do not provide enough choices regarding polymer types and pore sizes. Most importantly, commercially available inserts are two-dimensional multiporous membrane-based devices. Herein, we report a one-step fabrication process of the multifunctional nanofiber-based permeable hanging cell culture insert using electrospinning. These fabricated nanofibrous membranes' attached inserts have advantages such as low cost, ready availability, easy fabrication, tunable porosity, autoclavability, and biomaterial-based nanofibrous membranes. The inserts without nanofibrous membrane can also be reused by autoclaving them and electrospun nanofibrous membrane on it according to the application. We have also confirmed its suitability for extensive use in the field of cell culture by analyzing its adherence and toxicity results on breast cancer cell line (MCF-7). These hanging cell culture inserts are thus a potent product for various cell culture assays such as cell migration for wound healing, cancer metastasis, and other tissue engineering applications.
悬挂式细胞培养插入物是应用最为广泛的细胞培养装置，可为各种共培养和三培养系统提供独立的多室设置。除了成本高昂外，市售的插入物在聚合物类型和孔径方面没有提供足够的选择。最重要的是，市售的插入物是基于二维多孔膜的装置。在此，我们报告了一种使用静电纺丝一步法制造基于多功能纳米纤维的可渗透悬挂式细胞培养插入物的过程。这些带有附着的纳米纤维膜的插入物具有低成本、易于获得、易于制造、孔隙率可调、可高压灭菌以及基于生物材料的纳米纤维膜等优点。没有纳米纤维膜的插入物也可以通过高压灭菌并根据应用需求在其上静电纺丝纳米纤维膜来重复使用。我们还通过分析其对乳腺癌细胞系（MCF-7）的黏附性和毒性结果，证实了其在细胞培养领域广泛应用的适用性。因此，这些悬挂式细胞培养插入物是用于各种细胞培养测定的有力产品，如用于伤口愈合、癌症转移的细胞迁移以及其他组织工程应用。
