Rennick R E, Campbell J H, Campbell G R
Baker Medical Research Institute, Prahran, Victoria, Australia.
Atherosclerosis. 1988 May;71(1):35-43. doi: 10.1016/0021-9150(88)90300-0.
The effect of macrophages on rabbit vascular smooth muscle phenotype and proliferative ability was examined using ultrastructural morphometry. The volume fraction of myofilaments (Vv myo) in smooth muscle cells (SMC) from 9-week-old rabbit aorta in vivo was 39.5 +/- 1.2%. After seeding the enzymatically isolated SMC at 4 X 10(5) cells/ml in primary culture, the Vv myo was 38.9 +/- 1.2% on day 3 dropping to 29.9 +/- 2.0% by day 5. On day 6 the Vv myo was 29.2 +/- 1.8%, and the cells began to proliferate. Confluency was reached after less than 24 h proliferation and the Vv myo rose abruptly on day 7 to 36.9 +/- 1.9%. When the SMC were co-cultured with macrophages, the Vv myo fell to 31.2 +/- 0.9% on day 3 and to 25.9 +/- 0.5% on day 5 at which time cells commenced proliferation. Confluency occurred on day 6 but the SMC Vv myo did not rise throughout the rest of the culture period (27.4 +/- 1.8% and 26.9 +/- 1.3% on days 7 and 9, respectively) and the cells, unlike the controls, continued to proliferate, becoming multi-layered. Early phenotypic modulation in sparsely seeded SMC (8 X 10(4) cells/ml) co-cultured with macrophages was also found using fluorescent labelled antibodies to smooth muscle myosin. Measurement of proliferation by cell counts (and tritiated thymidine autoradiography) showed that macrophages stimulated SMC in primary culture to proliferate at a significantly greater rate than control cells grown alone in 5% whole blood serum (WBS). Proliferation of subcultured SMC co-cultured with macrophages was also stimulated.(ABSTRACT TRUNCATED AT 250 WORDS)
采用超微结构形态计量学方法研究了巨噬细胞对兔血管平滑肌表型和增殖能力的影响。体内9周龄兔主动脉平滑肌细胞（SMC）中肌丝的体积分数（Vv myo）为39.5±1.2%。将酶分离的SMC以4×10⁵个细胞/ml接种于原代培养，第3天Vv myo为38.9±1.2%，到第5天降至29.9±2.0%。第6天Vv myo为29.2±1.8%，细胞开始增殖。增殖不到24小时后达到汇合，第7天Vv myo突然升至36.9±1.9%。当SMC与巨噬细胞共培养时，第3天Vv myo降至31.2±0.9%，第5天降至25.9±0.5%，此时细胞开始增殖。第6天出现汇合，但在培养期剩余时间内SMC的Vv myo未升高（第7天和第9天分别为27.4±1.8%和26.9±1.3%），并且与对照不同，细胞继续增殖，形成多层。使用平滑肌肌球蛋白荧光标记抗体还发现，与巨噬细胞共培养的稀疏接种SMC（8×10⁴个细胞/ml）存在早期表型调节。通过细胞计数（和氚标记胸腺嘧啶核苷放射自显影）测量增殖表明，巨噬细胞刺激原代培养中的SMC增殖，其速率显著高于在5%全血血清（WBS）中单独生长的对照细胞。与巨噬细胞共培养的传代培养SMC的增殖也受到刺激。（摘要截短于250字）
