新冠疫情中无症状传播的系统发育基因组溯源

Phylogenomic tracing of asymptomatic transmission in a COVID-19 outbreak.

作者信息

Zhang Ju, Ding Nan, Song Yangzi, Song Rui, Pan Yang, Wang Linghang, Yan Shuo, Wang Qi, Ma Shanfang, Wei Lirong, Yu Fengting, Lu Lianhe, Zhang Fujie, Chen Chen, Zeng Hui

机构信息

Beijing Ditan Hospital, Capital Medical University, Beijing 100015, China.

Beijing Key Laboratory of Emerging Infectious Diseases, Beijing 100015, China.

出版信息

Innovation (Camb). 2021 May 28;2(2):100099. doi: 10.1016/j.xinn.2021.100099. Epub 2021 Mar 22.

SARS-CoV-2 has caused over 100 million deaths and continues to spread rapidly around the world. Asymptomatic transmission of SARS-CoV-2 is the Achilles' heel of COVID-19 public health control measures. Phylogenomic data on SARS-CoV-2 could provide more direct information about asymptomatic transmission. In this study, using a novel MINERVA sequencing technology, we traced asymptomatic transmission of COVID-19 patients in Beijing, China. One hundred and seventy-eight close contacts were quarantined, and 14 COVID-19 patients were laboratory confirmed by RT-PCR. We provide direct phylogenomic evidence of asymptomatic transmission by constructing the median joining network in the cluster. These data could help us to determine whether the current symptom-based screening should cover asymptomatic persons.

严重急性呼吸综合征冠状病毒2（SARS-CoV-2）已导致超过1亿人死亡，且仍在全球迅速传播。SARS-CoV-2的无症状传播是新冠疫情公共卫生控制措施的致命弱点。SARS-CoV-2的系统基因组数据可以提供有关无症状传播的更直接信息。在本研究中，我们使用一种新型的MINERVA测序技术，追踪了中国北京新冠患者的无症状传播情况。178名密切接触者被隔离，14名新冠患者通过逆转录聚合酶链反应（RT-PCR）得到实验室确诊。我们通过构建聚类中的中介连接网络，提供了无症状传播的直接系统基因组证据。这些数据可以帮助我们确定当前基于症状的筛查是否应涵盖无症状者。