Zhou Jingwei, Singh Ravi P, Ren Yong, Bai Bin, Li Zhikang, Yuan Chan, Li Shunda, Huerta-Espino Julio, Liu Demei, Lan Caixia
Huazhong Agricultural University, College of Plant Science & Technology, No. 1, Hongshan District, Wuhan 430070, Hubei Province, P.R. China.
International Maize and Wheat Improvement Center (CIMMYT), 06600 Mexico D.F., Mexico.
Plant Dis. 2021 Nov;105(11):3705-3714. doi: 10.1094/PDIS-12-20-2654-RE. Epub 2021 Nov 18.
The characterization of leaf rust (caused by ) and stripe rust (caused by f. sp. ) resistance genes is the basis for breeding resistant wheat varieties and managing epidemics of these diseases in wheat. A cross between the susceptible wheat variety 'Apav#1' and resistant variety 'Neimai 836' was used to develop a mapping population containing 148 F recombinant inbred lines (RILs). Leaf rust phenotyping was done in field trials at Ciudad Obregón, Mexico, in 2017 and 2018, and stripe rust data were generated at Toluca, Mexico, in 2017 and in Mianyang, Ezhou, and Gansu, China, in 2019. Inclusive complete interval mapping (ICIM) was used to create a genetic map and identify significant resistance quantitative trait loci (QTL) with 2,350 polymorphic markers from a 15K wheat single-nucleotide polymorphism (SNP) array and simple-sequence repeats (SSRs). The pleiotropic multipathogen resistance gene and four QTL were identified, including two new loci, and , which explained 3 to 16% of the phenotypic variation in resistance to leaf rust and 7 to 14% of that to stripe rust. The flanking SNP markers for the two loci were converted to Kompetitive Allele-Specific PCR (KASP) markers and used to genotype a collection of 153 wheat lines, indicating the Chinese origin of the loci. Our results suggest that Neimai 836, which has been used as a parent for many wheat varieties in China, could be a useful source of high-level resistance to both leaf rust and stripe rust.
叶锈病（由 引起）和条锈病（由 f. sp. 引起）抗性基因的鉴定是培育抗小麦品种和控制小麦这些病害流行的基础。利用感病小麦品种“Apav#1”与抗病品种“内麦836”杂交，构建了一个包含148个F重组自交系（RILs）的作图群体。2017年和2018年在墨西哥奥布雷贡市进行田间试验对叶锈病进行表型分析，2017年在墨西哥托卢卡以及2019年在中国绵阳、鄂州和甘肃对条锈病数据进行测定。采用完备区间作图法（ICIM），利用来自15K小麦单核苷酸多态性（SNP）芯片和简单序列重复（SSR）的2350个多态性标记构建遗传图谱并鉴定显著的抗性数量性状位点（QTL）。鉴定出了多效性多病原菌抗性基因 和4个QTL，包括2个新位点 和 ，它们解释了叶锈病抗性表型变异的3%至16%以及条锈病抗性表型变异的7%至14%。将这两个位点的侧翼SNP标记转化为竞争性等位基因特异性PCR（KASP）标记，并用于对153个小麦品系进行基因分型，表明这些位点起源于中国。我们的结果表明，在中国已被用作许多小麦品种亲本的内麦836可能是叶锈病和条锈病高水平抗性的有用来源。
