National Institute of Agricultural and Livestock Researches (INIAP-Ecuador), Santa Catalina Experimental Station, Quito, Ecuador; and Colegio de Postgraduados (CP), Campus Montecillo, Montecillo, Texcoco 56230, State of México, México.
Campo Experimental Valle de México INIFAP, 56230 Chapingo, State of México, México.
Plant Dis. 2018 Feb;102(2):421-427. doi: 10.1094/PDIS-11-16-1545-RE. Epub 2017 Dec 18.
Since 1984, the 'Chilero' spring wheat line developed by CIMMYT has proven to be highly resistant to leaf rust and stripe rust. Amid efforts to understand the basis of resistance of this line, a recombinant inbred line (RIL) population derived from a cross between Avocet and Chilero was studied. The parents and RILs were characterized in field trials for leaf rust and stripe rust in three locations in Mexico between 2012 and 2015 and genotyped with DArT-array, DArT-GBS, and SSR markers. A total of 6,168 polymorphic markers were used to construct genetic linkage maps. Inclusive composite interval mapping detected four colocated resistance loci to both rust diseases and two stripe rust resistant loci in the Avocet × Chilero population. Among these, the quantitative trait locus (QTL) on chromosome 1BL was identified as a pleotropic adult plant resistance gene Lr46/Yr29, whereas QLr.cim-5DS/QYr.cim-5DS was a newly discovered colocated resistance locus to both rust diseases in Chilero. Additionally, one new stripe rust resistance locus on chromosome 7BL was mapped in the current population. Avocet also contributed two minor colocated resistance QTLs situated on chromosomes 1DL and 4BS. The flanking SNP markers can be converted to breeder friendly Kompetitive Allele Specific PCR (KASP) markers for wheat breeding programs.
自 1984 年以来，CIMMYT 开发的“Chilero”春小麦品系被证明对叶锈病和条锈病具有高度抗性。在努力了解该品系抗性基础的过程中，研究了源自 Avocet 和 Chilero 杂交的重组自交系（RIL）群体。在 2012 年至 2015 年期间，在墨西哥的三个地点对亲本和 RIL 进行了田间叶锈病和条锈病的鉴定，并利用 DArT-array、DArT-GBS 和 SSR 标记进行了基因型鉴定。共使用了 6168 个多态性标记来构建遗传连锁图谱。包容性复合区间作图检测到 Avocet×Chilero 群体中对两种锈病的四个共定位抗性基因座和两个条锈病抗性基因座。其中，染色体 1BL 上的数量性状基因座（QTL）被鉴定为广谱成株期抗性基因 Lr46/Yr29，而 QLr.cim-5DS/QYr.cim-5DS 是 Chilero 中对两种锈病的新发现的共定位抗性基因座。此外，在当前群体中还定位到了 7BL 上的一个新的条锈病抗性基因座。Avocet 还在染色体 1DL 和 4BS 上贡献了两个较小的共定位抗性 QTL。侧翼 SNP 标记可以转化为小麦育种计划中使用的育性友好型 Kompetitive Allele Specific PCR（KASP）标记。
