159397Huashan Hospital Fudan University, Shanghai, China.
J Biomater Appl. 2021 Aug;36(2):210-218. doi: 10.1177/0885328221995926. Epub 2021 Mar 28.
Rupture of the rotator cuff is a common injury of the shoulder joint in sports professionals. In addition, research on repair of the rotator cuff has gained popularity over the recent years. Given the high rate of re-tear after surgery, it is necessary to design and prepare biodegradable materials with good mechanical properties, for the management of the condition. Consequently, the present study conducted surface modification of the simvastatin factor-loaded silk fibroin for the repair of chronic rotator cuff injury in SD rats. The experiments were analyzed through scanning electron microscopy and the water contact angle. Additionally, the CCK-8 assay was used to observe the effect of the intervention on the proliferation of BMSCs. Moreover, the osteogenic differentiation of BMSCs was detected through the ALP and ARS assays while the expression of osteogenic genes was examined using qRT-PCR and Western blot analysis. Furthermore, a model for repairing chronic rotator cuff tears in SD rats was established . Thereafter, rotator cuff repair and healing were evaluated through HE staining while Masson and Sirius staining was used to detect the collagen formation ratio. Additionally, the study analyzed the mechanism underlying the effect of simvastatin-loaded silk fibroin. The results showed that the simvastatin-loaded silk fibroin membrane had better biocompatibility and the experiments confirmed that it could promote the proliferation and osteogenic differentiation of BMSCs. In addition, the HE staining experiments similarly confirmed that it could enhance tendon bone healing and alleviate inflammation in chronic rotator cuff injuries. On the other hand, Masson and Sirius staining showed that the simvastatin-loaded silk fibroin could promote the formation of collagen. Further analysis also revealed that it could promote the osteogenic differentiation of BMSCs by activating the β-catenin signaling pathway. In general, these findings suggested that surface modification of the simvastatin factor-loaded silk fibroin was a potential means of improving the healing of rotator cuff injuries and can be implemented in clinical practice in future.
肩袖撕裂是运动专业人员常见的肩关节损伤。此外，近年来对肩袖修复的研究也越来越受到关注。鉴于手术后再次撕裂的高发生率，有必要设计和准备具有良好机械性能的可生物降解材料，以对这种情况进行管理。因此，本研究对载辛伐他汀的丝素蛋白进行表面改性，用于修复 SD 大鼠慢性肩袖损伤。通过扫描电子显微镜和水接触角分析实验。此外，通过 CCK-8 测定法观察干预对 BMSCs 增殖的影响。而且，通过碱性磷酸酶（ALP）和抗酒石酸酸性磷酸酶（ARS）测定法检测 BMSCs 的成骨分化，通过 qRT-PCR 和 Western blot 分析检测成骨基因的表达。此外，建立了修复 SD 大鼠慢性肩袖撕裂的模型。然后，通过 HE 染色评估肩袖修复和愈合情况，通过 Masson 和 Sirius 染色检测胶原形成比例。此外，研究分析了载辛伐他汀丝素蛋白作用的机制。结果表明，载辛伐他汀丝素蛋白膜具有更好的生物相容性，实验证实其可以促进 BMSCs 的增殖和成骨分化。此外，HE 染色实验同样证实，它可以增强肌腱骨愈合，减轻慢性肩袖损伤的炎症。另一方面，Masson 和 Sirius 染色表明，载辛伐他汀丝素蛋白可以促进胶原的形成。进一步分析还表明，它可以通过激活β-连环蛋白信号通路促进 BMSCs 的成骨分化。总之，这些发现表明，载辛伐他汀丝素蛋白的表面改性是改善肩袖损伤愈合的一种潜在方法，将来可以在临床实践中实施。
