de Bono D P, Pittilo M, Pringle S, Green C
Cardiovascular Research Unit, University of Edinburgh, UK.
Br J Exp Pathol. 1988 Apr;69(2):209-20.
The interactions of cultured bovine aortic and human umbilical or saphenous vein endothelium with cultured fibroblasts or smooth muscle cells were studied using light microscopy, scanning electron microscopy, and a radioisotope adhesion assay. (1) Resuspended fibroblasts or smooth muscle cells readily 'overgrew' confluent endothelial monolayers under static culture conditions, but not when cultures were exposed to a continuously stirred medium. (2) Exposure of cultured endothelial or smooth muscle cells to a moderately alkaline environment alters the disposition of pericellular concanavalin. A positive extracellular material. This does not affect the initial adhesion of endothelial or smooth muscle cells, but does affect cell spreading. (3) Endothelial adhesion to cultured smooth muscle cells involves both adhesion and spreading. Recently subcultured or rapidly proliferating smooth muscle cells support initial adhesion, but not spreading. Spreading appears to require the establishment of a suitable extracellular matrix, and this is inhibited both by a flowing medium and by an alkaline extracellular environment.
利用光学显微镜、扫描电子显微镜和放射性同位素黏附试验，研究了培养的牛主动脉内皮细胞、人脐静脉内皮细胞或大隐静脉内皮细胞与培养的成纤维细胞或平滑肌细胞之间的相互作用。（1）在静态培养条件下，重悬的成纤维细胞或平滑肌细胞很容易“生长过度”，覆盖汇合的内皮细胞单层，但当培养物暴露于持续搅拌的培养基中时则不会。（2）将培养的内皮细胞或平滑肌细胞暴露于中等碱性环境会改变细胞周围伴刀豆球蛋白（一种阳性细胞外物质）的分布。这并不影响内皮细胞或平滑肌细胞的初始黏附，但会影响细胞铺展。（3）内皮细胞与培养的平滑肌细胞的黏附涉及黏附和铺展。最近传代培养或快速增殖的平滑肌细胞支持初始黏附，但不支持铺展。铺展似乎需要建立合适的细胞外基质，而流动的培养基和碱性细胞外环境均会抑制这一过程。
