Metabolomics Research Group, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo s/n, 40170-115, Salvador, Brazil.
Departamento de Bioquímica, Instituto de Química, Universidade de São Paulo, Av. Prof. Lineu Prestes, 748, 05508-000, São Paulo, Brazil.
Chem Biodivers. 2021 May;18(5):e2100055. doi: 10.1002/cbdv.202100055. Epub 2021 Apr 21.
Despite its importance as a medicinal plant, there is a lack of studies that assessed the chemical composition of A. cochliacarpos extracts. Herein, we used a metabolite profiling approach and chemometrics as a powerful strategy to correlate the chemical composition with the antioxidant activity of A. cochliacarpos extracts. Extracts obtained with ethyl acetate showed greater antioxidant activity and higher total phenolic content than extracts obtained with hexane. The chemical composition was assessed by HPLC/HR-MS and it encompassed fatty alcohols, terpenoids, phenolic derivatives, lipids, carotenoid-like compounds, alkaloids, flavonoids, polyketides, and glycerophospholipids. Chemometrics successfully differentiated not only the chemical composition of extracts in response to the nature of the extraction solvent and the botanical part used during extraction but also it allowed us to associate the chemical composition with the antioxidant activity of the extracts, which might be particularly helpful for drug discovery and development programs.
尽管作为一种药用植物很重要，但仍缺乏评估 A. cochliacarpos 提取物化学成分的研究。在这里，我们使用代谢物分析方法和化学计量学作为一种强大的策略，将化学成分与 A. cochliacarpos 提取物的抗氧化活性相关联。用乙酸乙酯提取的提取物比用正己烷提取的提取物具有更高的抗氧化活性和更高的总酚含量。通过 HPLC/HR-MS 评估化学成分，包括脂肪醇、萜类化合物、酚类衍生物、脂质、类胡萝卜素化合物、生物碱、类黄酮、聚酮和甘油磷脂。化学计量学不仅成功地区分了提取物的化学成分，以响应提取溶剂的性质和提取过程中使用的植物部分，而且还使我们能够将化学成分与提取物的抗氧化活性相关联，这对于药物发现和开发计划可能特别有帮助。
