Metabolomics Research Group, Instituto de Química, Universidade Federal da Bahia, Rua Barão de Jeremoabo s/n, 40170-115 Salvador, Brazil.
Laboratório de Neuroquímica e Biologia Celular, Departamento de Bioquímica e Biofísica, Instituto de Ciências da Saúde, Universidade Federal da Bahia, Salvador, Brazil.
Food Chem. 2021 Dec 1;364:130453. doi: 10.1016/j.foodchem.2021.130453. Epub 2021 Jun 24.
Lepidium meyenii is an edible plant that has been used as a nutritional supplement worldwide due to its medicinal properties. However, most of the studies have focused on the pharmacological activities of the extracts rather than their chemical composition. Herein, we used a combination of a multiplatform metabolite profiling approach and chemometrics to identify bioactive metabolites in L. meyenii. Extracts obtained with ethyl acetate and ethanol showed the promising antioxidant, anti-glioma and antibacterial activities. Sixty metabolites were identified by HPLC-MS, whereas fifteen were identified by GC-MS. Partial least squares discriminant analysis (PLS-DA), hierarchical cluster analysis (HCA), and Variable Importance in Projection (VIP) successfully discriminated extracts obtained in different organic solvents from in natura dry roots and commercial product samples of L. meyenii. Additionally, correlation analysis allowed us to pinpoint potential candidates responsible for each biological activity tested for the extracts, which could be extrapolate for other food-related species.
莱菔子是一种可食用的植物，由于其药用特性，已在全球范围内用作营养补充剂。然而，大多数研究都集中在提取物的药理活性上，而不是其化学成分上。在此，我们采用多平台代谢物分析方法和化学计量学相结合的方法，鉴定莱菔子中的生物活性代谢物。用乙酸乙酯和乙醇提取的提取物具有有希望的抗氧化、抗神经胶质瘤和抗菌活性。通过 HPLC-MS 鉴定出 60 种代谢物，通过 GC-MS 鉴定出 15 种代谢物。偏最小二乘判别分析（PLS-DA）、层次聚类分析（HCA）和变量重要性投影（VIP）成功地区分了用不同有机溶剂从干根和莱菔子商业产品中提取的提取物。此外，相关性分析使我们能够确定每个提取物的每种生物活性的潜在候选物质，这可以外推到其他与食品相关的物种。
