Department of Neurosurgery, First Hospital of Jilin University, Changchun, Jilin, China.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei, Taiwan.
J Appl Biomater Funct Mater. 2021 Jan-Dec;19:22808000211005379. doi: 10.1177/22808000211005379.
The goal of this study is to understand the ability of a newly developed barrier membrane to enhance bone tissue regeneration. Here in this study we present the in vitro characterization of the barrier membrane made from type I collagen and crosslinked by oligomeric proanthocyanidins (OPCs). The effects of the membrane (P-C film) on cell cycle, proliferation, alkaline phosphatase activity, and mineralization were evaluated using the human osteoblast cell line MG-63, while the barrier ability was examined using MG-63 cells, as well as the human skin fibroblast cell line WS-1. The pore size is one of the factors that plays a key role in tissue regeneration, therefore, we evaluated the pore size of the membrane using a capillary flow porometer. Our results showed that the mean pore size of the P-C film was approximately 7-9 µm, the size known to inhibit cell migration across the membrane. The P-C film also demonstrated excellent cell viability and good biocompatibility, since the cell number increased with time, with MG-63 cells proliferating faster on the P-C film than in the cell culture flask. Furthermore, the P-C film promoted osteoblast differentiation, resulting in higher alkaline phosphatase activity and mineralization. Therefore, our results suggest that this P-C film has a great potential to be used in guided bone regeneration during periodontal regeneration and bone tissue engineering.
本研究旨在探讨一种新型屏障膜在促进骨组织再生方面的能力。本研究中，我们介绍了由 I 型胶原蛋白和低聚原花青素（OPCs）交联而成的屏障膜的体外特性。采用人成骨肉瘤细胞系 MG-63 评估了该膜（P-C 膜）对细胞周期、增殖、碱性磷酸酶活性和矿化的影响，同时采用 MG-63 细胞和人皮肤成纤维细胞系 WS-1 评估了其屏障能力。孔径是影响组织再生的关键因素之一，因此我们使用毛细管流动孔隙度仪评估了膜的孔径。结果表明，P-C 膜的平均孔径约为 7-9μm，这一尺寸已知可抑制细胞穿过膜的迁移。P-C 膜还表现出良好的细胞活力和生物相容性，因为细胞数量随时间增加，MG-63 细胞在 P-C 膜上的增殖速度快于细胞培养瓶。此外，P-C 膜促进成骨细胞分化，导致碱性磷酸酶活性和矿化增加。因此，我们的结果表明，这种 P-C 膜在牙周再生和骨组织工程中的引导骨再生中具有很大的应用潜力。
