Department of Neurosurgical Oncology, First Hospital, Jilin University, Changchun, China.
Department of Chemical Engineering and Biotechnology, National Taipei University of Technology (Taipei Tech), Taipei, Taiwan.
Mater Sci Eng C Mater Biol Appl. 2019 Aug;101:640-649. doi: 10.1016/j.msec.2019.03.112. Epub 2019 Apr 10.
In this study, type I collagen membranes were prepared using oligomeric proanthocyanidins (OPCs) as the cross-linking agent. The fabricated materials were evaluated to be applied as guided tissue regeneration membranes for periodontal defects. The mechanical strength of the cross-linked collagen membranes, namely OPCs-Col films, using different concentrations of OPCs ranged from 30 to 60 kPa. The cross-linked collagen membranes had better thermal stability than non-cross-linked one and could effectively resist the decomposition in collagenase solution as long as fifty days. The results of material characterization showed that 10% OPCs-Col film was ideal for our purpose. In vitro study using L929 and MG-63 cells revealed that 10% OPCs-Col film had great biocompatibility while OPC was demonstrated to be not cytotoxic as glutaraldehyde and genipin but even promote L929 cells. The material was further studied for in vivo studies with two models, subcutaneous and cranium defects in rat. The subcutaneous test showed that the regeneration membrane degraded till one month and the inflammatory response also reduced with implantation time. When implanted into the cranium defect, no lesions of the brain were caused and new bone tissue was observed inside the material. The results of in vivo studies showed that the synthesized membrane was helpful for tissue regeneration with long degradation time. The tissue regeneration membranes can barrier the rapid growing soft tissue, in order to save the capacity for the growth of neo bone.
在这项研究中，使用低聚原花青素（OPC）作为交联剂制备了 I 型胶原膜。评估了所制备的材料作为牙周缺损引导组织再生膜的应用。不同浓度 OPC 交联的胶原膜（即 OPCs-Col 膜）的机械强度范围为 30 至 60kPa。交联胶原膜比非交联的具有更好的热稳定性，并且只要五十天就能有效抵抗胶原酶溶液中的分解。材料特性的结果表明，10%的 OPCs-Col 膜是最理想的。体外使用 L929 和 MG-63 细胞的研究表明，10%的 OPCs-Col 膜具有良好的生物相容性，而 OPC 被证明不像戊二醛和京尼平那样具有细胞毒性，甚至可以促进 L929 细胞的生长。该材料进一步在两种模型（大鼠皮下和颅骨缺损）中进行了体内研究。皮下试验表明，再生膜在一个月内降解，炎症反应也随着植入时间的延长而减轻。当植入颅骨缺损时，不会对脑组织造成损伤，并且可以观察到材料内部有新的骨组织。体内研究结果表明，合成的膜有助于组织再生，并且具有较长的降解时间。组织再生膜可以阻挡快速生长的软组织，以节省新骨生长的能力。
