Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland.
Clin Oral Investig. 2013 Apr;17(3):981-8. doi: 10.1007/s00784-012-0764-7. Epub 2012 Jun 6.
Bioresorbable collagen membranes are routinely utilized in guided bone regeneration to selectively direct the growth and repopulation of bone cells in areas of insufficient volume. However, the exact nature by which alveolar osteoblasts react to barrier membranes as well as the effects following the addition of growth factors to the membranes are still poorly understood. The objective of the present study was therefore to investigate the effect of a bioresorbable collagen membrane soak-loaded in growth factors bone morphogenetic protein 2 (BMP2) or transforming growth factor β1 (TGFβ1) on osteoblast adhesion, proliferation, and differentiation.
Prior to experimental seeding, membranes were soaked in either BMP2 or TGFβ1 at a concentration of 10 ng/ml for 5 min.
Human osteoblasts adhered to all soak-loaded membranes as assessed by scanning electron microscopy. Growth factors BMP2 and TGFβ1 increased osteoblast proliferation at 3 or 5 days post-seeding when compared to control collagen membranes. Analysis of real-time PCR revealed that administration of BMP2 increased osteoblast differentiation markers such as osterix, collagen I, and osteocalcin. BMP2 also increased mineralization of primary osteoblasts as demonstrated by alizarin red staining when compared to control and TGFβ1 soak-loaded membranes.
The combination of a collagen barrier membrane with growth factors TGFβ1 and BMP2 significantly influenced adhesion, proliferation, and differentiation of primary human osteoblasts.
The described in vitro effects following the combination of collagen barrier membranes with growth factors TGFβ1 and BMP2 provide further biologic support for the clinical application of this treatment strategy in guided bone regeneration procedures.
生物可吸收胶原膜通常用于引导骨再生，以有选择性地引导骨细胞在体积不足的区域生长和再定植。然而，成骨细胞对屏障膜的反应的确切性质以及向膜中添加生长因子后的效果仍知之甚少。本研究的目的因此是研究生长因子骨形态发生蛋白 2（BMP2）或转化生长因子β1（TGFβ1）负载在生物可吸收胶原膜上对成骨细胞黏附、增殖和分化的影响。
在实验播种之前，将膜在浓度为 10ng/ml 的 BMP2 或 TGFβ1 中浸泡 5 分钟。
扫描电子显微镜评估表明，所有浸泡的膜均能黏附人成骨细胞。与对照胶原膜相比，生长因子 BMP2 和 TGFβ1 在接种后 3 或 5 天增加了成骨细胞的增殖。实时 PCR 分析显示，BMP2 处理增加了成骨细胞分化标志物，如osterix、I 型胶原和骨钙素。与对照和 TGFβ1 浸泡膜相比，BMP2 还增加了原代成骨细胞的矿化，通过茜素红染色显示。
胶原屏障膜与生长因子 TGFβ1 和 BMP2 的联合使用显著影响原代人成骨细胞的黏附、增殖和分化。
胶原屏障膜与生长因子 TGFβ1 和 BMP2 联合使用后的体外描述的作用为这种治疗策略在引导骨再生程序中的临床应用提供了进一步的生物学支持。
