Matsumoto A, Murakami S, Arai Y
Department of Anatomy, Juntendo University School of Medicine, Tokyo, Japan.
Cell Tissue Res. 1988 Apr;252(1):33-7. doi: 10.1007/BF00213823.
The preoptic area (POA) or cerebral cortex taken from newborn female rats were transplanted into the third ventricle of ovariectomized adult rats. From the day of transplantation, estradiol-17 beta in a silastic capsule was implanted subcutaneously into host animals for 4 weeks. The POA or cerebral cortex transplants were examined at light- and electron-microscopic levels 4 weeks after transplantation. All of the POA or cortical grafts showed an appearance similar to normal neural tissue. Estrogen exposure for 4 weeks via the host induced a significant increase in the volume of the POA grafts. The neuronal population of the POA grafts exposed to estrogen was not significantly different from that of the POA grafts without estrogen treatment. However, the number of axodendritic shaft and spine synapses of the POA grafts exposed to estrogen was significantly greater than that of the POA grafts without estrogen treatment. In contrast, there was no significant difference in the volume of the cortical tissues transplanted into the brain between the control and estrogen-treated groups. These results suggest that estrogen has a stimulatory effect on the development of neuronal substrates in the intraventricular POA graft, increasing its volume and synaptic population.
将新生雌性大鼠的视前区（POA）或大脑皮层移植到去卵巢成年大鼠的第三脑室。从移植当天起，将含有17β-雌二醇的硅橡胶胶囊皮下植入宿主动物体内，持续4周。在移植后4周，对视前区或大脑皮层移植物进行光镜和电镜检查。所有视前区或皮层移植物均呈现出与正常神经组织相似的外观。通过宿主给予4周的雌激素暴露导致视前区移植物的体积显著增加。暴露于雌激素的视前区移植物的神经元数量与未接受雌激素处理的视前区移植物相比无显著差异。然而，暴露于雌激素的视前区移植物的轴突-树突干和棘突触数量显著多于未接受雌激素处理的视前区移植物。相比之下，对照组和雌激素处理组移植到脑内的皮层组织体积无显著差异。这些结果表明，雌激素对脑室内视前区移植物中神经元底物的发育具有刺激作用，增加其体积和突触数量。
