Guo Shuqi, Fei Qiang
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
Shaanxi Key Laboratory of Energy Chemical Process Intensification, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2021 Mar 25;37(3):816-830. doi: 10.13345/j.cjb.200639.
Due to abundant availability of shale gas and biogas, methane has been considered as one of the most potential carbon sources for industrial biotechnology. Methanotrophs carrying the native methane monooxygenase are capable of using methane as a sole energy and carbon source, which provides a novel strategy for reducing greenhouse gas emission and substituting edible substrates used in bioconversion processes. With the rapid development of genetic engineering tools and biosynthesis techniques, various strategies for improving the efficiency of methane bioconversion have been achieved to produce a variety of commodity bio-based products. Herein, we summarize several important aspects related with methane utilization and metabolic engineering of methanotrophs, including the modification of methane oxidation pathways, the construction of efficient cell factories, and biosynthesis of chemicals and fuels. Finally, the prospects and challenges of the future development of methane bioconversion are also discussed.
由于页岩气和沼气的丰富供应，甲烷已被视为工业生物技术最具潜力的碳源之一。携带天然甲烷单加氧酶的甲烷氧化菌能够将甲烷作为唯一的能量和碳源，这为减少温室气体排放以及替代生物转化过程中使用的可食用底物提供了一种新策略。随着基因工程工具和生物合成技术的快速发展，已经实现了多种提高甲烷生物转化效率的策略，以生产各种基于生物的商品。在此，我们总结了与甲烷利用和甲烷氧化菌代谢工程相关的几个重要方面，包括甲烷氧化途径的修饰、高效细胞工厂的构建以及化学品和燃料的生物合成。最后，还讨论了甲烷生物转化未来发展的前景和挑战。
