Wang Weiting, Jiao Ziyue, Hou Qianzi, Guo Shuqi, Fei Qiang
School of Chemical Engineering and Technology, Xi'an Jiaotong University, Xi'an 710049, Shaanxi, China.
Xi'an Key Laboratory of C1 Compound Bioconversion Technology, Xi'an 710049, Shaanxi, China.
Sheng Wu Gong Cheng Xue Bao. 2024 Sep 25;40(9):2866-2883. doi: 10.13345/j.cjb.240102.
The utilization of C1 gases (CH, CO, and CO) for the production of oleochemicals applied in the energy and platform chemicals through microbial engineering has emerged as a promising approach to reduce greenhouse gas emissions and decrease dependence on fossil fuel. C1 gas-utilizing microorganisms, such as methanotrophs, microalgae, and acetogens, are capable of converting C1 gases as the sole substrates for cell growth and oleochemical synthesis with different carbon-chain lengths, garnering considerable attention from both scientific community and industry field for sustainable biomanufacturing. This paper comprehensively reviews recent advancements in the development of engineered cell factories utilizing C1 gases for the production of oleochemicals, elucidating the key metabolic pathways of biosynthesis. Furthermore, this paper highlights the research progress and prospects in optimizing gene expression, metabolic pathway reconstruction, and fermentation conditions for efficient oleochemical production from C1 gases. This review provides valuable insights and guidance for the efficient utilization of C1 gases and the development of carbon cycling-based bioeconomy.
通过微生物工程利用C1气体（CH、CO和CO）生产应用于能源和平台化学品的油脂化学品，已成为减少温室气体排放和降低对化石燃料依赖的一种有前景的方法。利用C1气体的微生物，如甲烷营养菌、微藻和产乙酸菌，能够将C1气体作为细胞生长和不同碳链长度油脂化学品合成的唯一底物，在可持续生物制造方面引起了科学界和工业界的广泛关注。本文全面综述了利用C1气体生产油脂化学品的工程细胞工厂开发的最新进展，阐明了生物合成的关键代谢途径。此外，本文重点介绍了在优化基因表达、代谢途径重建和发酵条件以从C1气体高效生产油脂化学品方面的研究进展和前景。本综述为C1气体的高效利用和基于碳循环的生物经济发展提供了有价值的见解和指导。
