Department of Chemical Engineering (Integrated Engineering), Kyung Hee University, Gyeonggi-do 17104, Republic of Korea.
Department of Chemical Engineering (Integrated Engineering), Kyung Hee University, Gyeonggi-do 17104, Republic of Korea.
Biotechnol Adv. 2021 Mar-Apr;47:107700. doi: 10.1016/j.biotechadv.2021.107700. Epub 2021 Feb 3.
Methane, the predominant element in natural gas and biogas, represents a promising alternative to carbon feedstocks in the biotechnological industry due to its low cost and high abundance. The bioconversion of methane to value-added products can enhance the value of gas and mitigate greenhouse gas emissions. Methanotrophs, methane-utilizing bacteria, can make a significant contribution to the production of various valuable biofuels and chemicals from methane. Type II methanotrophs in comparison with Type I methanotrophs have distinct advantages, including high acetyl-CoA flux and the co-incorporation of two important greenhouse gases (methane and CO), making it a potential microbial cell-factory platform for methane-derived biomanufacturing. Herein, we review the most recent advances in Type II methanotrophs related to multi-omics studies and metabolic engineering. Representative examples and prospects of metabolic engineering strategies for the production of suitable products are also discussed.
甲烷是天然气和沼气的主要成分，由于其成本低、丰度高，是生物技术行业中碳原料的一种很有前途的替代品。将甲烷生物转化为增值产品可以提高气体的价值并减少温室气体排放。甲烷利用细菌——甲烷营养菌，可以为从甲烷生产各种有价值的生物燃料和化学品做出重大贡献。与 I 型甲烷营养菌相比，II 型甲烷营养菌具有明显的优势，包括高乙酰辅酶 A 通量和两种重要温室气体（甲烷和 CO）的共掺入，使其成为一种潜在的基于微生物细胞的甲烷衍生生物制造平台。本文综述了与多组学研究和代谢工程相关的 II 型甲烷营养菌的最新进展。还讨论了生产合适产品的代谢工程策略的代表性实例和前景。
