Department of Gynaecology, The First People's Hospital of Fuyang, Hangzhou, China.
Department Gynaecology and Obstetrics, Jiading District Central Hospital Affiliated Shanghai University of Medicine & Health Sciences, Shanghai, China.
Cancer Biother Radiopharm. 2022 Nov;37(9):779-791. doi: 10.1089/cbr.2020.4184. Epub 2021 Mar 30.
Both microRNA (miR)-205 and GATA Binding Protein 3 () were involved in cervical cancer (CC), yet their correlation remained poorly understood. The authors' study aimed to unveil their correlation in CC. Clinical cervical tissue samples were collected. Survival rates of CC patients with high or low miR-205 and expressions were analyzed using Kaplan-Meier curve. CC cell viability, migration, and tube formation were measured by cell counting kit-8 assay, scratch assay, and tube formation assay, respectively. The potential binding sites between miR-205 and were predicted by TargetScan, and confirmed with dual-luciferase reporter assay. Relative expressions of miR-205, , vascular endothelial growth factor, E-cadherin, N-cadherin, and vimentin were quantified with quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed. MiR-205 was increased, yet was decreased in CC, indicating that they were negatively correlated. Upregulating miR-205 increased miR-205 expression and CC cell viability and promoted migration and tube formation, yet decreased expression, while downregulating miR-205 exerted the opposite effects. was the target gene of miR-205, and reversed the effect of miR-205 on expression and cell viability, migration, and tube formation in CC cells by reversing the effects of miR-205 on migration- and tube formation-related protein expressions. MiR-205 promotes CC cell viability, migration, and tube formation by targeting , providing new evidence for the implication of miR-205 in CC and a possible therapeutic method for CC. Clinical Trial Registration number: ZLK-20181103-01.
miR-205 和 GATA 结合蛋白 3 () 均参与宫颈癌 (CC) 的发生，但其相关性尚不清楚。本研究旨在揭示 miR-205 和 在 CC 中的相关性。收集临床宫颈组织标本。采用 Kaplan-Meier 曲线分析 miR-205 和 高表达或低表达的 CC 患者的生存率。通过细胞计数试剂盒-8 检测、划痕实验和管形成实验分别测量 CC 细胞活力、迁移和管形成。通过 TargetScan 预测 miR-205 和 之间的潜在结合位点，并通过双荧光素酶报告实验进行验证。根据需要用定量实时聚合酶链反应 (qRT-PCR) 和 Western blot 定量 miR-205、、血管内皮生长因子、E-钙黏蛋白、N-钙黏蛋白和波形蛋白的相对表达。miR-205 在 CC 中上调，而 下调，表明它们呈负相关。上调 miR-205 增加了 miR-205 的表达和 CC 细胞活力，并促进了迁移和管形成，而下调 miR-205 则产生相反的效果。 是 miR-205 的靶基因，通过逆转 miR-205 对迁移和管形成相关蛋白表达的影响，逆转 miR-205 对 表达和细胞活力、迁移和管形成的影响。miR-205 通过靶向 促进 CC 细胞活力、迁移和管形成，为 miR-205 参与 CC 提供了新的证据，并为 CC 提供了一种可能的治疗方法。临床试验注册号：ZLK-20181103-01。
