Department of Radiation Oncology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Department of Pathology, The First Affiliated Hospital of Soochow University, Suzhou, China.
Bioengineered. 2021 Dec;12(1):1066-1077. doi: 10.1080/21655979.2021.1899532.
In this study, we construct a subcutaneous tumor mice model of U14 cells, observe the tumor growth, and detect the expression of Foxp3 and VISTA in cervical cancer tissues and adjacent tissues during CMNa-enhancing radiotherapy.From the 15th day, compared with the control group, the tumor volume changes in each treatment group were significant (P < 0.01). CMNa combined with radiotherapy had an interactive effect and a positive effect in inhibiting tumor volume growth. There was no significant difference in the expression of Foxp3 and VISTA in mouse cervical cancer tissues and adjacent tissues in each group. The Foxp3 level in the RT group was the highest, and the CMNa group was the lowest. The VISTA level of the CMNa+RT group was the highest, the RT group is followed by, and the Control group is the lowest. The Foxp3 level of the CMNa group did not change much at each different point. The Foxp3 level in RT and CMNa+RT group gradually decreased after a transient increase, and the VISTA level in the CMNa+RT group increased more.Our results show that CMNa can enhance the efficacy of radiotherapy, and at the same time can reduce the compensatory increase in regulatory T cell Foxp3 levels caused by radiotherapy, and reduce the radiotherapy response. However, in the course of the treatment of the two, there may be a substantial increase in the level of VISTA, and the combined application of VISTA inhibitors may increase the anti-tumor response.
在这项研究中，我们构建了 U14 细胞的皮下肿瘤小鼠模型，观察肿瘤生长情况，并检测宫颈癌组织和相邻组织中 Foxp3 和 VISTA 的表达情况。在第 15 天，与对照组相比，各治疗组的肿瘤体积变化均有显著差异（P<0.01）。CMNa 联合放疗具有协同作用，能显著促进肿瘤体积生长抑制。各组小鼠宫颈癌组织和相邻组织中 Foxp3 和 VISTA 的表达无明显差异。RT 组 Foxp3 水平最高，CMNa 组最低。CMNa+RT 组 VISTA 水平最高，RT 组次之，对照组最低。CMNa 组 Foxp3 水平在各不同时间点变化不大。RT 和 CMNa+RT 组的 Foxp3 水平在短暂增加后逐渐下降，CMNa+RT 组的 VISTA 水平增加较多。我们的研究结果表明，CMNa 可以增强放疗的疗效，同时可以减少放疗引起的调节性 T 细胞 Foxp3 水平的代偿性增加，降低放疗反应。但在两种治疗方法的过程中，VISTA 水平可能会大幅升高，联合应用 VISTA 抑制剂可能会增加抗肿瘤反应。
