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生物陶瓷根尖充填材料微渗漏的体外评价:一项分光光度法研究。

In-vitro evaluation of microleakage of bioceramic root-end filling materials: A spectrophotometric study.

作者信息

Gupta Ravi, Kewalramani Radhika

机构信息

Department of Conservative Dentistry, Faculty of Dentistry, Melaka Manipal Medical College (Manipal Campus), Manipal Academy of Higher Education, Manipal, 576104, Karnataka, India.

NMC Medical Centre, Dubai, United Arab Emirates.

出版信息

J Oral Biol Craniofac Res. 2021 Apr-Jun;11(2):330-333. doi: 10.1016/j.jobcr.2021.03.001. Epub 2021 Mar 10.

DOI:10.1016/j.jobcr.2021.03.001
PMID:33786296
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7994504/
Abstract

AIM

To evaluate the microleakage of newer bioceramic root-end filling materials.

MATERIAL AND METHOD

Sixty freshly extracted human single-rooted mandibular premolar teeth were selected for the study. Teeth with fractured root, cracks, anddilacerations were rejected. All teeth were cleaned with ultrasonic scalers. Standard access opening was done and root canal treatment was performed with rotary files followed by obturation. After storing in saline for a week apical 3 ​mm of the root was resected at 900 angles to the long axis of the root. Retro cavity preparation was done with ultrasonic tips. The teeth were divided into four groups of 15 specimens each. Group I - Biodentin, GroupII-Bioaggregate, Group III - MTA Plus, and Group IV - MTA. After the restoration of retro cavities of all the teeth as per manufacture instructions, two coats of nail varnish were applied to leave apical 3 ​mm. All teeth were stored in 2% methylene blue for 72 ​h followed by emersion in 65% nitric acid for the next 72 ​h for Dye extraction. The obtained supernatant solution was then centrifuged and optical density or absorbance was measured with a UV spectrophotometer.

RESULT

Microleakage was found to be increasing in this order: Biodentin ​< ​Bioaggregate ​< ​MTA Plus ​< ​MTA. No significant difference was found in the microleakage of Biodentin compared to that of Bioaggregate(p ​> ​0.01).

CONCLUSION

All materials exhibit some amount of microleakage. Biodentin shows the least microleakage among all the bioceramic material groups. Hence, Biodentin and bioaggregate are better material of choice for the retrograde filling to prevent microleakage.

摘要

目的

评估新型生物陶瓷根充材料的微渗漏情况。

材料与方法

选取60颗新鲜拔除的人单根下颌前磨牙用于本研究。排除有牙根折断、裂纹和弯曲的牙齿。所有牙齿均用超声洁牙器清洁。进行标准开髓,并用旋转锉进行根管治疗,随后进行根管充填。在盐水中储存一周后,将牙根根尖3毫米处与牙根长轴呈90°角切除。用超声头制备倒凹洞形。将牙齿分为四组,每组15个标本。第一组 - 生物牙本质,第二组 - 生物聚集体,第三组 - MTA Plus,第四组 - MTA。按照制造商说明对所有牙齿的倒凹洞形进行修复后,涂两层指甲油,留出根尖3毫米未涂。所有牙齿在2%亚甲蓝中储存72小时,然后在接下来的72小时浸入65%硝酸中进行染料提取。然后将获得的上清液离心,并用紫外分光光度计测量光密度或吸光度。

结果

发现微渗漏按此顺序增加:生物牙本质<生物聚集体<MTA Plus<MTA。与生物聚集体相比,生物牙本质的微渗漏无显著差异(p>0.01)。

结论

所有材料均表现出一定程度的微渗漏。在所有生物陶瓷材料组中,生物牙本质的微渗漏最少。因此,生物牙本质和生物聚集体是用于逆行充填以防止微渗漏的更好选择材料。

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