Aksel Hacer, Küçükkaya Eren Selen, Askerbeyli Õrs Sevinc, Karaismailoğlu Eda
Hacettepe University, Faculty of Dentistry, Department of Endodontics, Ankara, Turkey.
Kastamonu University, Faculty of Medicine, Department of Biostatistics, Kastamonu, Turkey.
J Appl Oral Sci. 2018 Dec 10;27:e20180093. doi: 10.1590/1678-7757-2018-0093.
Surface changes in biological environments are critical for the evaluation of physical and biological activity of biomaterials. Objective: This study investigated surface alterations of calcium silicate-based cements after exposure to different environments.
Material and Methods: Forty-eight cylindrical cavities were prepared on root surfaces. The cavities were filled using ProRoot MTA or Biodentine and assigned to four subgroups (n=6): dry, wet, acidic, and blood. Surface topographies were evaluated using an optical profilometer for 28 days, and the roughness of the material surfaces was quantified. Vertical dimensional change was measured by determining the height difference between the material surface and the flat tooth surface. Data were compared with a two-way repeated measures ANOVA and Bonferroni tests.
Results: In dry condition, the surface roughness of MTA or Biodentine was constant up to 3 days (p>0.05) but decreased after 28 days (p<0.05). In dry condition, ProRoot MTA presented constant surface level through time, while Biodentine showed decreased surface level after 28 days. In wet condition, the roughness and the surface levels of both materials increased after 1 day (p<0.05). Neither the surface roughness nor the levels of the materials showed significant changes in acidic conditions (p>0.05). Both materials showed the highest roughness in blood conditions on the 1st day (p<0.05), while the surface roughness in blood decreased dramatically after 28 days. The roughness of Biodentine was higher in wet conditions up to 3 days compared with ProRoot MTA (p<0.05). Likewise, in blood condition, Biodentine showed higher roughness on the 28th day than ProRoot MTA (p<0.05).
Conclusions: Dry, wet, and blood conditions had a time-dependent effect on the surface roughness and vertical dimensional changes of the materials. However, acidic conditions did not affect the roughness and the surface level of the materials.
生物环境中的表面变化对于评估生物材料的物理和生物活性至关重要。目的：本研究调查了硅酸钙基水门汀在暴露于不同环境后的表面变化。
材料与方法：在牙根表面制备48个圆柱形窝洞。使用ProRoot MTA或Biodentine填充窝洞，并分为四个亚组（n = 6）：干燥、湿润、酸性和血液环境。使用光学轮廓仪评估表面形貌，为期28天，并对材料表面粗糙度进行量化。通过测定材料表面与平坦牙面之间的高度差来测量垂直尺寸变化。数据采用双向重复测量方差分析和Bonferroni检验进行比较。
结果：在干燥条件下，MTA或Biodentine的表面粗糙度在3天内保持恒定（p>0.05），但在28天后降低（p<0.05）。在干燥条件下，ProRoot MTA的表面水平随时间保持恒定，而Biodentine在28天后表面水平降低。在湿润条件下，两种材料的粗糙度和表面水平在1天后均增加（p<0.05）。在酸性条件下，材料的表面粗糙度和水平均未显示出显著变化（p>0.05）。两种材料在血液环境中第1天的粗糙度最高（p<0.05），而在血液环境中28天后表面粗糙度急剧下降。在长达3天的湿润条件下，Biodentine的粗糙度高于ProRoot MTA（p<0.05）。同样，在血液环境中，Biodentine在第28天的粗糙度高于ProRoot MTA（p<0.05）。
结论：干燥、湿润和血液环境对材料的表面粗糙度和垂直尺寸变化具有时间依赖性影响。然而，酸性条件不影响材料的粗糙度和表面水平。
