Department of Clinical Laboratory, Tianjin First Central Hospital, School of Medicine, Nankai University, Tianjin, China.
TEDA School of Biological Sciences and Biotechnology, Nankai University, Tianjin, 300192, China.
Ann N Y Acad Sci. 2021 Aug;1497(1):74-90. doi: 10.1111/nyas.14595. Epub 2021 Mar 30.
Raoultella ornithinolytica is a poorly understood opportunistic pathogen, and the underlying mechanisms of its multidrug resistance and pathogenicity have not yet been comprehensively investigated. The multidrug-resistant (MDR) strain WM1 was isolated from the blood of a male patient in Tianjin, China, in 2018. Here, we describe the complete genome and provide a genomic analysis of R. ornithinolytica WM1. The isolate was resistant to all tested antimicrobials except amikacin, tobramycin, and tigecycline. Two plasmids, pWM1-1 (IncHI5) and pWM1-2 (IncR), carried multidrug-resistance regions. A large antimicrobial resistance island region resided on pWM1-1 and exhibited mosaic structures resulting from the acquisition of complex integrations of variable regions, including genes conferring resistance to multiple classes of antimicrobials. Moreover, WM1 possessed virulence-related elements that encode several virulence factors, including type I fimbriae, Escherichia coli common pilus, type II and VI secretion systems, yersiniabactin, enterobactin, and surface polysaccharide, indicating pathogenic potential. Furthermore, the core genome phylogeny and pan-genome analyses revealed extensive genetic diversity. Our analysis indicates the need for stringent infection control, antimicrobial stewardship, periodic resistance monitoring, and rational medication to address potential threats posed by MDR R. ornithinolytica strains.
鸟氨酸罗单胞菌是一种了解甚少的机会性病原体，其多药耐药性和致病性的潜在机制尚未得到全面研究。耐多药（MDR）菌株 WM1 于 2018 年从中国天津的一名男性患者血液中分离得到。在这里，我们描述了完整的基因组，并对鸟氨酸罗单胞菌 WM1 进行了基因组分析。该分离株对除阿米卡星、妥布霉素和替加环素以外的所有测试抗菌药物均有耐药性。两个质粒，pWM1-1（IncHI5）和 pWM1-2（IncR），携带多药耐药区域。一个大型的抗菌药物耐药岛区域位于 pWM1-1 上，并表现出马赛克结构，这是由于可变区域的复杂整合而导致的，包括赋予对多种类抗菌药物耐药的基因。此外，WM1 具有与毒力相关的元素，编码多种毒力因子，包括 I 型菌毛、大肠杆菌普通菌毛、II 型和 VI 型分泌系统、耶尔森菌素、肠杆菌素和表面多糖，表明其具有致病性潜力。此外，核心基因组系统发育和泛基因组分析显示出广泛的遗传多样性。我们的分析表明，需要严格的感染控制、抗菌药物管理、定期耐药监测和合理用药，以应对 MDR 鸟氨酸罗单胞菌菌株带来的潜在威胁。
