The State Key Laboratory Breeding Base of Basic Science of Stomatology (Hubei-MOST) & Key Laboratory of Oral Biomedicine Ministry of Education, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Department of Orthodontics, School & Hospital of Stomatology, Wuhan University, Wuhan, China.
Eur J Oral Sci. 2021 Jun;129(3):e12784. doi: 10.1111/eos.12784. Epub 2021 Mar 30.
In this study, we evaluated the anti-biofilm and anti-demineralization abilities of a novel material, CMC-ClyR-ACP nanogel, designed by loading the chimeric lysin ClyR and amorphous calcium phosphate (ACP) into a nanocarrier material carboxymethyl chitosan (CMC), in a demineralization model. Dynamic light scattering, transmission electron microscopy, and Fourier transmission infrared spectroscopy showed that CMC-ClyR-ACP nanogel was synthesized successfully. Enamel samples prepared from premolars were divided into five groups according to their treatments with: (i) double distilled water ddH O, (ii) CMC-ACP, (iii) CMC-ClyR-ACP, (iv) ClyR, or (v) 0.12% chlorhexidine. Streptococcus mutans was allowed to form biofilms on the teeth for two days before treatment procedures were carried out from day 3 to day 6. The relative biofilm viability analyzed by Cell Counting Kit-8 showed that it was significantly lower (at 55.7%) for CMC-ClyR-ACP than seen for ddH O (89.9%), which was consistent with result of confocal laser scanning microscopy. The percentage surface hardness loss of CMC-ClyR-ACP (29.2%) was significantly lower than that of CMC-ACP (51.0%) and ClyR (58.7%) alone, and there was no significant difference between CMC-ClyR-ACP and chlorhexidine (26.9%), which was confirmed by scanning electron microscopy. Therefore, CMC-ClyR-ACP nanogel may be an effective strategy for the control of enamel demineralization.
在这项研究中，我们评估了一种新型材料 CMC-ClyR-ACP 纳米凝胶的抗生物膜和抗脱矿能力，该材料通过将嵌合溶菌酶 ClyR 和无定形磷酸钙 (ACP) 载入纳米载体材料羧甲基壳聚糖 (CMC) 来设计。动态光散射、透射电子显微镜和傅里叶变换红外光谱表明成功合成了 CMC-ClyR-ACP 纳米凝胶。根据处理方法，将从前磨牙制备的牙釉质样本分为五组：(i) 双蒸水 ddH2O、(ii) CMC-ACP、(iii) CMC-ClyR-ACP、(iv) ClyR 或 (v) 0.12% 洗必泰。从第 3 天到第 6 天，在处理程序进行之前，让变形链球菌在牙齿上形成生物膜两天。细胞计数试剂盒-8 分析的相对生物膜活力表明，CMC-ClyR-ACP 组（55.7%）明显低于 ddH2O 组（89.9%），这与共聚焦激光扫描显微镜的结果一致。CMC-ClyR-ACP（29.2%）的表面硬度损失百分比明显低于 CMC-ACP（51.0%）和 ClyR（58.7%）单独使用的情况，与洗必泰（26.9%）相比没有显著差异，扫描电子显微镜证实了这一点。因此，CMC-ClyR-ACP 纳米凝胶可能是控制牙釉质脱矿的有效策略。
