Laboratorio de Gametos y Desarrollo Tecnológico, Facultad de Estudios Superiores Iztacala, UNAM. Paseo de los Barrios Número 1, Tlalnepantla, Estado de México, Mexico.
Posgrado en Ciencias Biológicas, Unidad de Posgrado, Edificio A, Circuito de posgrados, 1er piso, Ciudad Universitaria, Ciudad de México, Mexico.
Cytometry A. 2021 Oct;99(10):999-1006. doi: 10.1002/cyto.a.24343. Epub 2021 Apr 5.
Sperm motility and concentration are commonly evaluated parameters in semen analysis. Those parameters are assessed objectively with commercial instrumentation such as computer-assisted sperm analysis systems (CASA) and hemocytometer. In CASA systems, sperm motility is assessed in the horizontal plane imposed by the stage of the microscope. Thus, there is lack of measurement of the vertical velocity of sperm. The female reproductive tract is a tridimensional space which the sperm traverse to reach the ovum, and there is a need for instruments measuring parameters more relevant to this real-world situation. In this report we describe the design, construction and use of an open-source hardware (OSH) device for evaluation of the vertical velocity of sperm, called UPSPERM. This device was also used to measure sperm concentration, and agreement with hemocytometer was evaluated. Bland-Altman analysis shows good agreement between these two methods of sperm counting. As a first application of UPSPERM, we evaluated the changes in boar sperm motility at distinct pH values between 7.0 and 8.0. The UPSPERM results showed that the vertical velocity of sperm was highest at pH 7.6 and 7.8. We propose that our UPSPERM offers a reliable and affordable option for obtaining measurements of vertical velocity and sperm concentration.
精子活力和浓度通常是精液分析中评估的参数。这些参数可以通过商业仪器（如计算机辅助精子分析系统（CASA）和血球计数器）客观评估。在 CASA 系统中，精子活力在显微镜载物台产生的水平面上进行评估。因此，缺乏对精子垂直速度的测量。女性生殖道是一个三维空间，精子需要穿越这个空间才能到达卵子，因此需要能够测量更符合实际情况的参数的仪器。在本报告中，我们描述了一个开源硬件（OSH）设备的设计、构建和使用情况，该设备用于评估精子的垂直速度，称为 UPSPERM。该设备还可用于测量精子浓度，并评估与血球计数器的一致性。 Bland-Altman 分析显示这两种精子计数方法具有良好的一致性。作为 UPSPERM 的首次应用，我们评估了在 7.0 到 8.0 之间不同 pH 值下公猪精子活力的变化。UPSPERM 的结果表明，在 pH 值为 7.6 和 7.8 时，精子的垂直速度最高。我们提出，我们的 UPSPERM 为获得垂直速度和精子浓度的测量值提供了一种可靠且经济实惠的选择。
