Wu Xin, Wang Chen-Kai, Zuo Hai-Yan, Chen Zhao-Hui, Wu Sheng-Bing, Zhou Mei-Qi
School of Acupuncture and Massage,Anhui University of Chinese Medicine Hefei 230038,China Graduate School,Anhui University of Chinese Medicine Hefei 230038,China.
Graduate School,Anhui University of Chinese Medicine Hefei 230038,China.
Zhongguo Zhong Yao Za Zhi. 2021 Mar;46(5):1084-1093. doi: 10.19540/j.cnki.cjcmm.20201121.102.
In order to enrich the transcriptome data of Fagopyrum dibotrys plants, analyze the genes encoding key enzyme involved in flavonoid biosynthesis pathway, and mine their functional genes, in this study, we performed RNA sequencing analysis for the rhizomes, roots, flowers, leaves and stems of F. dibotrys on the BGISEQ-500 sequencing platform. After de novo assembly of transcripts, a total of 205 619 unigenes were generated and 132 372 unigenes were obtained and annotated into seven public databases, of which, 81 327 unigenes were mapped to the GO database and most of the unigenes were annotated in cellular process, biological regulation, binding and catalytic activity. Besides, 86 922 unigenes were enriched in 136 pathways using KEGG database' and we identified 82 unigenes that encodes key enzymes involved in flavonoid biosynthesis. Comparing rhizome with root, flower, leaf or stem in F. dibotrys, 27 962 co-expressed differentially expressed genes(DEGs) were obtained. Among them, 23 515 DEGs of rhizome tissue-specific were enriched into 132 pathways and 13 unigenes were significantly enriched in biosynthesis of flavone and flavonol. In addition, we also identified 3 427 unigenes encoding 60 transcription factor(TFs) families as well as four unigenes encoding bHLH TFs were enriched in flavonoid biosynthesis. Our results greatly enriched the transcriptome database of plants, provided a reference for the analysis of key enzymes involved in flavonoid biosynthesis in plants, and will facilitate the study of the functions and regulatory mechanisms of key enzymes involved in flavonoid biosynthesis in F. dibotrys at the genetic level.
为了丰富金荞麦植物的转录组数据，分析黄酮类生物合成途径中关键酶的编码基因，并挖掘其功能基因，本研究在BGISEQ-500测序平台上对金荞麦的根茎、根、花、叶和茎进行了RNA测序分析。转录本进行从头组装后，共生成205619个单基因，其中132372个单基因被注释到七个公共数据库中，其中81327个单基因被映射到GO数据库，且大多数单基因在细胞过程、生物调控、结合和催化活性方面得到注释。此外，利用KEGG数据库，86922个单基因富集到136条途径中，我们鉴定出82个编码黄酮类生物合成关键酶的单基因。比较金荞麦的根茎与根、花、叶或茎，获得了27962个共表达差异表达基因（DEG）。其中，根茎组织特异性的23515个DEG富集到132条途径中，13个单基因在黄酮和黄酮醇的生物合成中显著富集。此外，我们还鉴定出3427个编码60个转录因子（TF）家族的单基因，以及四个编码bHLH TF的单基因在黄酮类生物合成中富集。我们的结果极大地丰富了植物转录组数据库，为植物黄酮类生物合成关键酶的分析提供了参考，并将有助于在基因水平上研究金荞麦黄酮类生物合成关键酶的功能和调控机制。
