一株缺失负责肠杆菌共同抗原和鞭毛生物合成的62个基因的菌株的构建与应用

Construction and Application of an Strain Lacking 62 Genes Responsible for the Biosynthesis of Enterobacterial Common Antigen and Flagella.

作者信息

Qiao Jun, Tan Xin, Huang Danyang, Li Hedan, Wang Zhen, Ren Hongyu, Hu Xiaoqing, Wang Xiaoyuan

机构信息

State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China.

Key Laboratory of Industrial Biotechnology, Ministry of Education, School of Biotechnology, Jiangnan University, Wuxi 214122, China.

出版信息

J Agric Food Chem. 2021 Apr 14;69(14):4153-4163. doi: 10.1021/acs.jafc.1c00453. Epub 2021 Mar 31.

DOI:10.1021/acs.jafc.1c00453

PMID:33787256

Abstract

The biosynthesis of the enterobacterial common antigen and flagella in consumes lots of substrates and energy. In this study, 12 genes responsible for the biosynthesis of the enterobacterial common antigen were deleted in MG1655, resulting in WQM021. WQM021 grew better than MG1655 in both rich LB medium and minimum M9 medium. Compared with MG1655, WQM021 showed higher membrane permeability and higher production efficiency for recombinant proteins, polyhydroxyalkanoate, and l-threonine. Transcriptome analysis revealed that genes relevant to glucose consumption, glycolysis, and flagellar synthesis were significantly upregulated in WQM021. Therefore, 50 genes responsible for flagellar biosynthesis were further deleted in WQM021, resulting in WQM022. WQM022 grew better and could synthesize more polyhydroxyalkanoate and l-threonine than WQM021. The results demonstrate that the productivity of can be efficiently improved when the enterobacterial common antigen and flagella are eliminated. This strategy has guiding significance in the optimization of other industrial products and microorganisms.

摘要

肠杆菌共同抗原和鞭毛的生物合成消耗大量底物和能量。在本研究中，在MG1655中删除了12个负责肠杆菌共同抗原生物合成的基因，得到WQM021。WQM021在丰富的LB培养基和基本M9培养基中生长均优于MG1655。与MG1655相比，WQM021对重组蛋白、聚羟基脂肪酸酯和L-苏氨酸表现出更高的膜通透性和更高的生产效率。转录组分析表明，与葡萄糖消耗、糖酵解和鞭毛合成相关的基因在WQM021中显著上调。因此，在WQM021中进一步删除了50个负责鞭毛生物合成的基因，得到WQM022。WQM022生长更好，并且比WQM021能合成更多的聚羟基脂肪酸酯和L-苏氨酸。结果表明，消除肠杆菌共同抗原和鞭毛时，大肠杆菌的生产力可得到有效提高。该策略对其他工业产品和微生物的优化具有指导意义。