Department of Clinical Chemistry, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
Department of Oncology, St. Olavs Hospital, Trondheim University Hospital, Trondheim, Norway.
Scand J Clin Lab Invest. 2021 Jul;81(4):303-306. doi: 10.1080/00365513.2021.1904283. Epub 2021 Mar 31.
Presently, bed-side or at home quantification of neutrophils in blood (b-neutrophils) is not practical, because cytometric methods are too expensive and technically demanding. We have explored whether calprotectin concentration in whole blood (b-calprotectin) might be a valid measure of b-neutrophils because this principle might be used in a simple and robust immunoassay device. We obtained heparin blood samples from 77 patients with possible neutropenia, most of them cancer patients treated with cytostatic drugs, and compared b-calprotectin with their b-neutrophils in a simultaneously taken EDTA-blood sample. The Spearman rank correlation coefficient between b-calprotectin and b-neutrophils was 0.986 ( < .0001). In a regression model of b-neutrophils as a function of age, gender, type of hematology instrument, total leukocyte count minus neutrophils, b-calprotectin, and plasma calprotectin (p-calprotectin), only b-calprotectin was a statistically significant predictor. B-neutrophils below 1 × 10/L was unlikely if b-calprotectin was above 50 mg/L. In conclusion, b-calprotectin, without adjusting for p-calprotectin, correlates closely with b-neutrophils and could be used to detect b-neutrophils below 1 × 10/L.
目前，床边或家庭中血液中性粒细胞（b-中性粒细胞）的定量检测并不实际，因为细胞计数方法过于昂贵且技术要求高。我们探索了全血中钙卫蛋白（b-钙卫蛋白）浓度是否可以作为 b-中性粒细胞的有效衡量指标，因为该原理可能用于简单而强大的免疫分析设备中。我们从 77 名疑似中性粒细胞减少症患者（大多数为接受细胞毒性药物治疗的癌症患者）获得肝素血样，并在同时采集的 EDTA 血样中比较 b-钙卫蛋白与 b-中性粒细胞。b-钙卫蛋白与 b-中性粒细胞之间的 Spearman 秩相关系数为 0.986（ < .0001）。在 b-中性粒细胞作为年龄、性别、血液学仪器类型、白细胞总数减去中性粒细胞、b-钙卫蛋白和血浆钙卫蛋白（p-钙卫蛋白）的函数的回归模型中，只有 b-钙卫蛋白是具有统计学意义的预测因子。如果 b-钙卫蛋白高于 50mg/L，则 b-中性粒细胞低于 1×10/L的可能性不大。总之，b-钙卫蛋白无需调整 p-钙卫蛋白，与 b-中性粒细胞密切相关，可用于检测 b-中性粒细胞低于 1×10/L。
