Programa de Pós-Graduação em Engenharia de Processos, Universidade da Região de Joinville/UNIVILLE, Departamentos de Engenharia Química e de Farmácia, Rua Paulo Malschitzki, 10, 89219-710 Joinville, SC, Brazil.
Programa de Pós-Graduação em Engenharia Química, Universidade Federal de Santa Catarina (UFSC), CTC, Campus Universitário, Trindade, 88040-900 Florianópolis, SC, Brazil.
An Acad Bras Cienc. 2021 Mar 26;93(1):e20191153. doi: 10.1590/0001-3765202120191153. eCollection 2021.
Bisphenol A is an endocrine interfering compound, produced and used on a large scale worldwide. Chemical and biologic methods can be used to remove it from the environment. Biological methods are considered less costly, safer and, according to green chemistry definitions, an environmentally correct method. Considering the use of a crude enzyme broth, without any downstream process, the costs could be mostly reduced. Thus, the removal of bisphenol A by Pleurotus sajor-caju crude enzyme broth was investigated. Initially, the agro-industrial wastes were characterized and, the composition of the culture medium and the bioreactor culture conditions were defined. The enzyme produced in the highest concentration was characterized and the crude broth used in the bisphenol A removal assays. The OXI45 culture medium presented the highest laccase activity (1,850.7 U L-1, 350 rpm). Greater laccase stability was observed at 20 - 40 oC and pHs 5 - 7. Vanillin and ferulic acid (considered mediator compounds) were identified in the crude broth, probably helping on the obtention of the high value of removal effectiveness (0.052 mg U-1 h-1). The results indicate the potential use of the Pleurotus sajor-caju crude enzyme broth to obtain an enzymatic formulation for application in the environmental area.
双酚 A 是一种内分泌干扰化合物，在全球范围内大量生产和使用。可以使用化学和生物方法将其从环境中去除。生物方法被认为成本更低、更安全，并且根据绿色化学的定义，是一种正确的环境方法。考虑到使用未经任何下游处理的粗酶制剂，成本可以大大降低。因此，研究了糙皮侧耳粗酶制剂对双酚 A 的去除作用。首先，对农业工业废物进行了表征，并确定了培养基的组成和生物反应器的培养条件。对产酶量最高的酶进行了表征，并将粗酶制剂用于双酚 A 的去除实验。在 OXI45 培养基中，漆酶活性最高（1850.7 U/L，350 rpm）。在 20-40°C 和 pH5-7 条件下，漆酶稳定性更好。在粗酶制剂中鉴定出香草醛和阿魏酸（被认为是介体化合物），这可能有助于获得高去除效率（0.052 mg U-1 h-1）。结果表明，糙皮侧耳粗酶制剂具有潜在的应用价值，可用于获得环境领域应用的酶制剂。
