Universidade Federal de Pernambuco - UFPE, Research Centre for Therapeutic Innovation - Suely Galdino - NUPIT-SG, Laboratory of Immunomodulation and New Therapeutical Approaches, Recife, PE, Brasil.
Universidade Federal do Vale do São Francisco - UNIVASF, Medicine Collegiate, Paulo Afonso, BA, Brasil.
Braz J Biol. 2021 Mar 29;82:e236297. doi: 10.1590/1519-6984.236297. eCollection 2021.
Plectranthus barbatus Andrews (Lamiaceae) is widely distributed in the world and has a range of popular therapeutic indications. This work aimed to evaluate the phytochemical characterization of two leaf extracts of P. barbatus, and their antimicrobial, antineoplastic and immunomodulatory potential. After collection, herborization and obtainment of the P. barbatus aqueous extract (PBA) and acetone:water 7:3 P. barbatus organic extract (PBO), the phytochemical characterization was performed by high-performance liquid chromatography (HPLC). The antimicrobial activity was performed to determine the minimum inhibitory concentration (MIC) against eight bacterial strains using the microdilution test and the fungus Trichophyton rubrum by disc diffusion assay and microdilution test. Cytotoxicity was assessed by MTT and trypan blue methods in normal peripheral blood mononuclear cells (PBMCs) at concentrations ranged between 0.1 to 100 µg.mL-1 and in neoplastic cell lines Toledo, K562, DU-145 and PANC-1 at 1, 10 and 100 µg.mL-1 . Immunomodulatory activity, was evaluated by sandwich ELISA of proinflammatory cytokines at BALB/c mice splenocytes cultures supernatant. Both extracts presented flavonoids, cinnamic derivatives, steroids and ellagic acid. PBO showed bacteriostatic activity against Acinetobacter baumannii (MIC = 250 µg.mL-1) clinical isolate and PBA fungistatic activity against Trichophyton rubrum (MIC = 800 µg.mL-1). The extracts did not exhibit toxicity to PBMCs and neoplastic cells (IC50 > 100 µg.mL-1). Additionally, PBO at 100 µg.mL-1 significantly inhibited IFN-γ and IL-17A cytokines (p = 0.03). Plectranthus barbatus is a potential candidate for therapeutic use due to its low toxicity in healthy human cells and exhibits biological activities of medical interest as bacteriostatic, fungistatic and immunomodulatory.
普列兰属巴巴图斯（唇形科）广泛分布于世界各地，具有多种流行的治疗适应症。本工作旨在评价两种普列兰属巴巴图斯叶提取物的植物化学特征及其抗菌、抗肿瘤和免疫调节潜力。在采集、植物标本采集和获得普列兰属巴巴图斯水提取物（PBA）和丙酮：水 7：3 普列兰属巴巴图斯有机提取物（PBO）后，通过高效液相色谱法（HPLC）进行植物化学特征分析。通过微量稀释试验测定了八种细菌菌株的最小抑菌浓度（MIC），并用圆盘扩散法和微量稀释法测定了真菌红色毛癣菌。在浓度为 0.1 至 100 µg.mL-1 的正常外周血单核细胞（PBMC）和肿瘤细胞系 Toledo、K562、DU-145 和 PANC-1 中，通过 MTT 和台盼蓝法评估细胞毒性在 1、10 和 100 µg.mL-1 。通过 BALB/c 小鼠脾细胞培养上清液中促炎细胞因子的夹心 ELISA 评估免疫调节活性。两种提取物均含有类黄酮、肉桂衍生物、甾体和鞣花酸。PBO 对鲍曼不动杆菌（MIC = 250 µg.mL-1）临床分离株具有抑菌活性，PBA 对红色毛癣菌具有抑菌活性（MIC = 800 µg.mL-1）。提取物对 PBMC 和肿瘤细胞无毒性（IC50 > 100 µg.mL-1）。此外，PBO 在 100 µg.mL-1 时可显著抑制 IFN-γ 和 IL-17A 细胞因子（p = 0.03）。由于其对健康人类细胞的低毒性，普列兰属巴巴图斯可能是一种有治疗用途的候选药物，具有抑菌、抑菌和免疫调节等医学相关的生物活性。
