BGI Genomics, BGI-Shenzhen, Shenzhen, China.
Department of Physiology and Cell Biology, University of Nevada School of Medicine, Reno, NV, USA.
Biol Reprod. 2021 Jul 2;105(1):267-277. doi: 10.1093/biolre/ioab062.
Small noncoding RNAs deep sequencing (sncRNA-Seq) has become a routine for sncRNA detection and quantification. However, the software packages currently available for sncRNA annotation can neither recognize sncRNA variants in the sequencing reads, nor annotate all known sncRNA simultaneously. Here, we report a novel anchor alignment-based small RNA annotation (AASRA) software package (https://github.com/biogramming/AASRA). AASRA represents an all-in-one sncRNA annotation pipeline, which allows for high-speed, simultaneous annotation of all known sncRNA species with the capability to distinguish mature from precursor microRNAs, and to identify novel sncRNA variants in the sncRNA-Seq sequencing reads.
小非编码 RNA 深度测序 (sncRNA-Seq) 已成为 sncRNA 检测和定量的常规方法。然而，目前可用的 sncRNA 注释软件包既不能识别测序读段中的 sncRNA 变体，也不能同时注释所有已知的 sncRNA。在这里，我们报告了一种新的基于锚定比对的小 RNA 注释 (AASRA) 软件包 (https://github.com/biogramming/AASRA)。AASRA 代表了一种全功能的 sncRNA 注释管道，它允许以高速、同时注释所有已知的 sncRNA 物种，能够区分成熟和前体 microRNA，并识别 sncRNA-Seq 测序读段中的新 sncRNA 变体。
