Program in Food Science, Faculty of Food Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Program in Fermentation Technology in Food Industry, Faculty of Food Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok, 10520, Thailand.
Appl Biochem Biotechnol. 2021 Aug;193(8):2591-2601. doi: 10.1007/s12010-021-03553-5. Epub 2021 Mar 31.
In this study, we increased β-glucan production from brewer's yeast, Saccharomyces carlsbergensis RU01, by using tannic acid. High-pressure freezing and transmission electron microscopy (HPF-TEM) revealed that the yeast cell wall obtained from yeast malt (YM) medium supplemented with 0.1% w/v tannic acid was thicker than that of yeast cultured in YM medium alone. The production of β-glucan from S. carlsbergensis RU01 was optimized in 3% w/v molasses and 0.1% w/v diammonium sulfate (MDS) medium supplemented with 0.1% w/v tannic acid. The results showed that MDS medium supplemented with 0.1% w/v tannic acid significantly increased the dry cell weight (DCW), and the β-glucan production was 0.28±0.01% w/v and 11.99±0.04% w/w. Tannic acid enhanced the β-glucan content by up to 42.23%. β-Glucan production in the stirred tank reactor (STR) was 1.4-fold higher than that in the shake flask (SF) culture. Analysis of the β-glucan composition by Fourier transform infrared (FTIR) spectroscopy showed that the β-glucan of S. carlsbergensis RU01 cultured in MDS medium supplemented with 0.1% w/v tannic acid had a higher proportion of polysaccharide than that of the control. In addition, β-glucans from brewer's yeast can be used as prebiotic and functional foods for human health and in animal feed.
在这项研究中，我们使用单宁酸提高了卡尔酵母（Saccharomyces carlsbergensis RU01）的β-葡聚糖产量。高压冷冻和透射电子显微镜（HPF-TEM）显示，与仅在 YM 培养基中培养的酵母相比，从添加 0.1%（w/v）单宁酸的酵母麦芽（YM）培养基中获得的酵母细胞壁更厚。在 3%（w/v）糖蜜和 0.1%（w/v）硫酸二铵（MDS）培养基中优化了卡尔酵母（Saccharomyces carlsbergensis RU01）的β-葡聚糖生产，其中添加了 0.1%（w/v）单宁酸。结果表明，MDS 培养基中添加 0.1%（w/v）单宁酸可显著增加干细胞重量（DCW），β-葡聚糖产量为 0.28±0.01%（w/v）和 11.99±0.04%（w/w）。单宁酸使β-葡聚糖含量增加了 42.23%。搅拌罐反应器（STR）中的β-葡聚糖产量比摇瓶（SF）培养物高 1.4 倍。通过傅里叶变换红外（FTIR）光谱分析β-葡聚糖组成表明，在添加 0.1%（w/v）单宁酸的 MDS 培养基中培养的卡尔酵母（Saccharomyces carlsbergensis RU01）的β-葡聚糖中多糖的比例高于对照。此外，啤酒酵母的β-葡聚糖可用作人类健康和动物饲料的益生元和功能性食品。
