Vaithanomsat Pilanee, Boonlum Nutthamon, Trakunjae Chanaporn, Apiwatanapiwat Waraporn, Janchai Phornphimon, Boondaeng Antika, Phalinphattharakit Kanokwan, Nimitkeatkai Hataitip, Jarerat Amnat
Kasetsart Agricultural and Agro-Industrial Product Improvement Institute (KAPI), Kasetsart University, Chatuchak, Bangkok 10900, Thailand.
Food Technology Program, Mahidol University, Kanchanaburi Campus, Saiyok, Kanchanaburi 71150, Thailand.
Polymers (Basel). 2022 Apr 13;14(8):1582. doi: 10.3390/polym14081582.
β-Glucan (BG), one of the most abundant polysaccharides containing glucose monomers linked by β-glycosidic linkages, is prevalent in yeast biomass that needs to be recovered to obtain this valuable polymer. This study aimed to apply alkaline and enzymatic processes for the recovery of BG from the yeast strain TISTR 5925. For this purpose, the yeast was cultivated to produce the maximum yield of raw material (yeast cells). The effective recovery of BG was then established using either an alkaline or an enzymatic process. BG recovery of 35.45% was obtained by using 1 M NaOH at 90 °C for 1 h, and of 81.15% from 1% (/) hydrolytic protease enzyme at 55 °C for 5 h. However, BG recovered by the alkaline process was purer than that obtained by the enzymatic process. Fourier transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy confirmed the purity, the functional groups, and the linkages of BG obtained from different recovery systems and different raw materials. The results of this study suggest that an alkaline process could be an effective approach for the solubilization and recovery of considerable purity of BG from the yeast cells. In addition, the obtained BG had comparable functional properties with commercially available BG. This study reveals the effectiveness of both chemical and biological recovery of BG obtained from yeast as a potential polymeric material.
β-葡聚糖（BG）是最丰富的多糖之一，由β-糖苷键连接的葡萄糖单体组成，在酵母生物质中普遍存在，需要对其进行回收以获取这种有价值的聚合物。本研究旨在应用碱性和酶法从酵母菌株TISTR 5925中回收BG。为此，培养酵母以产生最大产量的原材料（酵母细胞）。然后使用碱性或酶法确定BG的有效回收率。在90℃下用1 M NaOH处理1小时，BG回收率为35.45%；在55℃下用1%（/）水解蛋白酶处理5小时，BG回收率为81.15%。然而，通过碱性方法回收的BG比通过酶法回收的更纯。傅里叶变换红外（FTIR）和核磁共振（NMR）光谱证实了从不同回收系统和不同原材料获得的BG的纯度、官能团和连接方式。本研究结果表明，碱性方法可能是从酵母细胞中溶解和回收相当纯度BG的有效方法。此外，所获得的BG与市售BG具有相当的功能特性。本研究揭示了从酵母中化学和生物回收BG作为潜在聚合物材料的有效性。
