Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Istanbul, Turkey.
Department of Food and Bioproduct Sciences, University of Saskatchewan, Saskatoon, Canada.
J Sci Food Agric. 2021 Oct;101(13):5707-5714. doi: 10.1002/jsfa.11225. Epub 2021 May 4.
In the presence of ascorbic acid, the degradation of acylated (sinapic, ferulic and p-coumaric acid derivatives of cyanidin-3-xylosylglucosylgalactoside) and non-acylated anthocyanins of black carrot extract (BCE) encapsulated in liposomes was studied. BCEs (0.2% and 0.4% w/w) were encapsulated in liposomes using different lecithin concentrations (1%, 2% and 4% w/w).
The liposomes were prepared with particle diameters of less than 50 nm and zeta potentials of about -21.3 mV for extract-containing liposomes and -27.7 mV for control liposomes. The encapsulation efficiency determined using high-performance liquid chromatography (HPLC) showed that increasing lecithin levels increased the efficiency to 59% at the same extract concentration. The concentrations of total anthocyanins and individual anthocyanins were determined for ascorbic acid (0.1% w/w)-degraded extract and liposomes (containing 0.2% w/w extract). Anthocyanin quantification of both liposomal and extract samples was performed by HPLC using cyanidin-3-O-glucoside chloride as standard. Five anthocyanins in the extract and encapsulated liposomes were quantified during 24 h (0-24 h): cyanidin-3-xylosylglucosylgalactoside 1.0-0.51 and 0.82-0.58 mg g , cyanidin-3-xylosylgalactoside 2.5-1.1 and 2.2-1.7 mg g , cyanidin-3-xylosyl(sinapoylglucosyl)galactoside 0.51-0.14 and 0.35-0.28 mg g , cyanidin-3-xylosyl(feruloylglucosyl)galactoside 1.37-0.41 and 1.06-0.98 mg g , and cyanidin-3-xylosyl(coumaroylglucosyl)galactoside 0.28-0.08 mg g for extract and 0.27-0.26 mg g for liposomes, respectively.
This study demonstrates the potential beneficial effect of liposomal encapsulation on individual, particularly acylated, anthocyanins after addition of ascorbic acid during a storage time of 24 h.
在抗坏血酸存在的情况下，研究了包封在脂质体中的酰化（芥子酸、阿魏酸和对香豆酸衍生物的矢车菊素-3-O-木糖基葡萄糖基半乳糖苷）和非酰化的黑胡萝卜提取物（BCE）的降解。BCE（0.2%和 0.4%w/w）用不同浓度的卵磷脂（1%、2%和 4%w/w）包封在脂质体中。
用高效液相色谱法（HPLC）测定包封效率，粒径小于 50nm，含提取物的脂质体的 zeta 电位约为-21.3mV，对照脂质体为-27.7mV。用 HPLC 测定包封效率表明，随着卵磷脂水平的增加，在相同提取物浓度下，包封效率增加到 59%。用高效液相色谱法（HPLC）测定了含有 0.1%w/w 抗坏血酸的提取物和脂质体（含 0.2%w/w 提取物）中总花色苷和各花色苷的浓度。用氰花定-3-O-葡萄糖氯酸盐作为标准，通过 HPLC 对脂质体和提取物样品中的花色苷进行定量分析。在 24 小时（0-24 小时）内对提取物和包封脂质体中的五种花色苷进行了定量分析：矢车菊素-3-O-木糖基葡萄糖基半乳糖苷 1.0-0.51 和 0.82-0.58mg/g，矢车菊素-3-O-木糖基半乳糖苷 2.5-1.1 和 2.2-1.7mg/g，矢车菊素-3-O-（芥子酰基葡萄糖基）半乳糖苷 0.51-0.14 和 0.35-0.28mg/g，矢车菊素-3-O-（阿魏酰基葡萄糖基）半乳糖苷 1.37-0.41 和 1.06-0.98mg/g，矢车菊素-3-O-（香豆酰基葡萄糖基）半乳糖苷 0.28-0.08mg/g 为提取物，0.27-0.26mg/g 为脂质体。
本研究表明，在 24 小时的储存时间内添加抗坏血酸后，脂质体包封对个体，特别是酰化的花色苷具有潜在的有益作用。
