Department of Food Engineering, Faculty of Chemical and Metallurgical Engineering, Istanbul Technical University, Maslak, Istanbul 34469, Turkey.
Department of Food Physics and Meat Science, Institute of Food Science and Biotechnology, University of Hohenheim, Garbenstrasse 21/25, Stuttgart 70599, Germany.
Food Res Int. 2018 Jun;108:491-497. doi: 10.1016/j.foodres.2018.03.071. Epub 2018 Mar 28.
In this study, the protection effect of black carrot extract on the stability of liposomes during storage was evaluated. The physical and chemical stability of the extract (0.4% w/w) including extract-loaded liposomes with various lecithin content (1%, 2%, and 4% w/w) were demonstrated. Z-average particle diameter and zeta potentials of liposomes were evaluated before and after 21 days of storage. The particle diameter of the samples was found to be lower than 50 nm after storage and no statistical change was determined (p < 0.05). The liposomes with and without extract were visualized by transmission electron microscopy (TEM) after negative staining. The TEM images revealed that unloaded and extract-loaded liposomes are similar in size. The degradation of extract (30%-90%), phenolic content (10%-29%), and antioxidant capacity (4%-33%) of liposomes depending on lecithin content were also determined during storage to evaluate the biochemical stability. A hexanal analysis was performed to investigate the lipid oxidation in liposomes within 60 days. According to the results, the oxidation of polyunsaturated fatty acids may be inhibited with the addition of the extract to liposomes; however, lipid content should be limited according to the extract concentration. The highest protection on oxidation was observed in extract-loaded liposomes containing 1% lecithin. The study provided valuable data on the contribution of an anthocyanin addition to liposomes to overcome oxidation of unsaturated phospholipids.
在这项研究中，评估了黑胡萝卜提取物对脂质体储存期间稳定性的保护作用。研究了提取物（0.4%w/w）和含有不同卵磷脂含量（1%、2%和 4%w/w）的提取物负载脂质体的物理和化学稳定性。在储存 21 天后，评估了脂质体的 Z 均粒径和 Zeta 电位。结果发现，储存后样品的粒径均低于 50nm，且无统计学差异（p<0.05）。用透射电子显微镜（TEM）对未经染色的载药和未载药脂质体进行了可视化观察。TEM 图像显示，未负载和负载提取物的脂质体大小相似。还在储存过程中测定了脂质体中提取物（30%-90%）、酚类含量（10%-29%）和抗氧化能力（4%-33%）的降解情况，以评估其生化稳定性。此外，还进行了己醛分析，以研究脂质体在 60 天内的脂质氧化情况。结果表明，添加提取物可能会抑制多不饱和脂肪酸的氧化；然而，应根据提取物浓度限制脂质含量。在含有 1%卵磷脂的负载提取物脂质体中观察到最高的氧化保护。该研究提供了有价值的数据，表明添加花色苷可克服不饱和磷脂的氧化，从而对脂质体起到保护作用。
