Chen Lu, Xu Wan-Li, Pei Li-Xia, Wu Xiao-Liang, Geng Hao, Guo Jing, Sun Jian-Hua
Department of Acupuncture-Moxibustion and Rehabilitation, Affiliated Hospital of Nanjing University of Chinese Medicine, Nanjing 210029, Jiangsu Province, China.
Zhongguo Zhen Jiu. 2021 Feb 12;41(2):137-41. doi: 10.13703/j.0255-2930.20200205-k0002.
To observe the clinical therapeutic effect of acupuncture therapy (acupuncture for regulating the mind and strengthening the spleen) on the gut microbiota and the content of fecal short-chain fatty acids (SCFAs) in patients with diarrhea type irritable bowel syndrome (IBS-D).
A total of 20 patients with IBS-D and 20 healthy subjects were included. The patients with IBS-D were treated with acupuncture therapy at Baihui (GV 20), Yintang (GV 29), Tianshu (ST 25), Shangjuxu (ST 37), Zusanli (ST 36), Sanyinjiao (SP 6) and Taichong (LR 3). The treatment was given once every two days, 3 times weekly, consecutively for 6 weeks. No intervention was provided for the healthy subjects. Before and after treatment, the IBS symptom severity score (IBS-SSS) was observed in the patients. The fecal samples were collected before and after treatment separately in the patients and in the healthy subjects. Using 16S rRNA sequencing technique, the structure and diversity of gut microbiota were analyzed. Using high-performance liquid chromatography, the content of SCFAs was detected.
After treatment, the abdominal pain severity score, the abdominal pain days score, the satisfaction in bowel movement score, the life interference score and the total IBS-SSS score were all lower than those before treatment in the patients with IBS-D (<0.05). Compared with the healthy subjects, Firmicutes was increased (<0.05), Bacteroidetes and Proteobacteria were decreased (<0.05), and Shannon index was reduced (<0.05) and Simpson index was increased in patients with IBS-D before treatment (<0.05). Compared before treatment, Firmicutes was decreased (<0.05), Bacteroidetes and Proteobacteria were increased (<0.05), and Shannon index was increased (<0.05) and Simpson index was reduced in the patients with IBS-D after treatment (<0.05). Besides, the content of SCFAs was reduced after treatment (<0.05).
acupuncture therapy relieves abdominal pain and diarrhea, which may be related to regulating the structure and diversity of gut microbiota and reducing the content of fecal SCFAs in the patients with IBS-D.
观察针刺疗法（调神健脾针法）对腹泻型肠易激综合征（IBS-D）患者肠道菌群及粪便短链脂肪酸（SCFAs）含量的临床治疗效果。
共纳入20例IBS-D患者和20例健康受试者。对IBS-D患者采用针刺百会（GV 20）、印堂（GV 29）、天枢（ST 25）、上巨虚（ST 37）、足三里（ST 36）、三阴交（SP 6）和太冲（LR 3）进行治疗。治疗每两天进行1次，每周3次，连续进行6周。对健康受试者不进行干预。观察患者治疗前后的IBS症状严重程度评分（IBS-SSS）。分别在患者和健康受试者治疗前后采集粪便样本。采用16S rRNA测序技术分析肠道菌群的结构和多样性。采用高效液相色谱法检测SCFAs的含量。
治疗后，IBS-D患者的腹痛严重程度评分、腹痛天数评分、排便满意度评分、生活干扰评分及IBS-SSS总分均低于治疗前（<0.05）。与健康受试者相比，IBS-D患者治疗前厚壁菌门增加（<0.05），拟杆菌门和变形菌门减少（<0.05），香农指数降低（<0.05），辛普森指数增加（<0.05）。与治疗前相比，IBS-D患者治疗后厚壁菌门减少（<0.05），拟杆菌门和变形菌门增加（<0.05），香农指数增加（<0.05），辛普森指数降低（<0.05）。此外，治疗后SCFAs含量降低（<0.05）。
针刺疗法可缓解腹痛和腹泻，这可能与调节IBS-D患者肠道菌群的结构和多样性以及降低粪便SCFAs含量有关。
