Aguilar-Lemarroy Adriana, López-Uribe Apolinar, Sánchez-Corona José, Jave-Suárez Luis Felipe
Division of Immunology, Western Biomedical Research Center, Mexican Social Security Institute, Guadalajara, Jalisco 44340, Mexico.
Exp Ther Med. 2021 May;21(5):485. doi: 10.3892/etm.2021.9916. Epub 2021 Mar 16.
The coronavirus disease 2019 (COVID-19) pandemic has become a serious global health problem and numerous studies are currently being conducted to improve understanding of the components of the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) virus, as well as to identify solutions that mitigate the effects of COVID-19 symptoms. The nutritional supplement Vita Deyun is composed of silymarin, glutathione, vitamin C and selenium. Studies of its individual components have demonstrated their benefits as anti-inflammatory agents, antioxidants and enhancers of the immune response. Therefore, the present study aimed to evaluate the effects of Vita Deyun on the expression of angiotensin-converting enzyme 2 () in diverse cell lines, as well as in the presence or absence of the SARS-CoV-2 open reading frame (ORF)3a protein. Through reverse transcription-quantitative PCR, the use of viral particles containing SARS-CoV-2 ORF3a and bioinformatics analysis via the National Center for Biotechnology Information databases, was determined to be highly expressed in oral and skin epithelial cells, with a lower expression observed in lung cells. Notably, the expression of SARS-CoV-2 ORF3a increased the level of expression and Vita Deyun treatment diminished this effect. In addition, Vita Deyun treatment markedly decreased interleukin-18 mRNA levels. The combination of phytonutrients in Vita Deyun may help to boost the immune system and could reduce the effects of COVID-19. Ongoing clinical studies are required to provide evidence of the efficacy of Vita Deyun.
2019年冠状病毒病（COVID-19）大流行已成为一个严重的全球健康问题，目前正在进行大量研究，以增进对严重急性呼吸综合征冠状病毒2（SARS-CoV-2）病毒成分的了解，并确定减轻COVID-19症状影响的解决方案。营养补充剂维她德运由水飞蓟宾、谷胱甘肽、维生素C和硒组成。对其单个成分的研究已证明它们作为抗炎剂、抗氧化剂和免疫反应增强剂的益处。因此，本研究旨在评估维她德运对多种细胞系中血管紧张素转换酶2（）表达的影响，以及在存在或不存在SARS-CoV-2开放阅读框（ORF）3a蛋白的情况下的影响。通过逆转录定量PCR、使用含有SARS-CoV-2 ORF3a的病毒颗粒以及通过国家生物技术信息中心数据库进行生物信息学分析，确定在口腔和皮肤上皮细胞中高表达，在肺细胞中表达较低。值得注意的是，SARS-CoV-2 ORF3a的表达增加了表达水平，而维她德运处理减弱了这种作用。此外，维她德运处理显著降低了白细胞介素-18 mRNA水平。维她德运中植物营养素的组合可能有助于增强免疫系统，并可能减轻COVID-19的影响。需要进行正在进行的临床研究以提供维她德运疗效的证据。
