细胞毒素相关基因A阳性通过抑制miR-125b-5p促进结肠癌细胞自噬。
Cytotoxin-Associated Gene A-Positive Promotes Autophagy in Colon Cancer Cells by Inhibiting miR-125b-5p.
作者信息
Zhong Xiaolin, Chen Ou, Zhou TieJun, Lü Muhan, Wan Juyi
机构信息
Department of Gastroenterology, The Affiliated Hospital of Southwest Medical University, Luzhou 646000, China.
Nuclear Medicine and Molecular Imaging Key Laboratory of Sichuan Province, Luzhou 646000, China.
出版信息
Can J Infect Dis Med Microbiol. 2021 Mar 2;2021:6622092. doi: 10.1155/2021/6622092. eCollection 2021.
OBJECTIVES
To investigate the effects of cytotoxin-associated gene A- (CagA-) positive on proliferation, invasion, autophagy, and expression of miR-125b-5p in colon cancer cells.
METHODS
Colon cancer cells were cocultured with (CagA+) to analyze the effects of on miR-125b-5p and autophagy. Colon cancer cells infected with (CagA+) were mimicked by transfection of CagA plasmid. The effects of CagA on the proliferation, invasion, and autophagy of colon cancer cells were analyzed. Cell counting kit-8 (CCK-8), clone formation, and Transwell assays were used to detect cell viability, proliferation, and invasion ability, respectively. Proteins and miRNAs were detected by western blotting and qPCR, respectively.
RESULTS
(CagA+) inhibited expression of miR-125b-5p and promoted autophagy in colon cancer cells. MiR-125 b-5p was underexpressed in colon cancer cells after CagA overexpression. CagA promoted colon cancer cell proliferation, invasion, and autophagy. Overexpression of miR-125b-5p inhibited the proliferation, invasion, and autophagy of colon cancer cells and reversed the effects of CagA.
CONCLUSION
(CagA+) infection may promote the development and invasion of colon cancer by inhibiting miR-125b-5p.
目的
探讨细胞毒素相关基因A（CagA）阳性对结肠癌细胞增殖、侵袭、自噬及miR-125b-5p表达的影响。
方法
将结肠癌细胞与CagA阳性菌株共培养，分析其对miR-125b-5p和自噬的影响。通过转染CagA质粒模拟感染CagA阳性的结肠癌细胞。分析CagA对结肠癌细胞增殖、侵袭和自噬的影响。分别采用细胞计数试剂盒-8（CCK-8）、克隆形成实验和Transwell实验检测细胞活力、增殖和侵袭能力。分别通过蛋白质印迹法和qPCR检测蛋白质和微小RNA。
结果
CagA阳性抑制结肠癌细胞中miR-125b-5p的表达并促进自噬。CagA过表达后，结肠癌细胞中miR-125b-5p表达下调。CagA促进结肠癌细胞的增殖、侵袭和自噬。miR-125b-5p过表达抑制结肠癌细胞的增殖、侵袭和自噬，并逆转CagA的作用。
结论
CagA阳性感染可能通过抑制miR-125b-5p促进结肠癌的发生和侵袭。
Zotero 插件