Nishikawa T, Kurihara S, Harada T, Sugawara M, Hatano H
Arch Dermatol Res (1975). 1977 Oct 27;260(1):1-6. doi: 10.1007/BF00558008.
The capability of complement fixation of pemphigus antibodies was tested using combined in vitro complement immunofluorescent (IF) staining methods. Three sera out of 25 serum samples from 22 pemphigus patients revealed positive reactions, while all other sera gave negative results. Specificity control tests confirmed the positive reactions to be specific for complement staining. Complement fixing pemphigus antibodies were titrated lower than corresponding IgG antibodies and were demonstrable only in the extensive stage of the disease. Thus, the present work supplied evidence that pemphigus antibodies fix complement in vitro. However, the discrepancy still remains between the in vivo deposition of complement in most cases of pemphigus and in vitro capability of complement fixation in only few cases. More investigations should be needed to explain the exact role of complement in pemphigus acantholysis.
采用体外补体免疫荧光（IF）联合染色方法检测天疱疮抗体的补体固定能力。来自22例天疱疮患者的25份血清样本中，有3份血清显示阳性反应，而其他所有血清均为阴性结果。特异性对照试验证实阳性反应对补体染色具有特异性。补体固定性天疱疮抗体的滴度低于相应的IgG抗体，且仅在疾病的广泛期可检测到。因此，本研究提供了天疱疮抗体在体外固定补体的证据。然而，在大多数天疱疮病例中补体的体内沉积与仅少数病例中的体外补体固定能力之间仍存在差异。需要更多的研究来解释补体在天疱疮棘层松解中的确切作用。
