Yang Minyan, Huang Guanyou, Qian Hua, Wang Jun, Zhao Shuyun, Li Shixiang, Yuan Yan
Hospital Affiliated 5 to Nantong University (Taizhou People's Hospital), Taizhou, China.
Reproductive Medicine Center, Department of Obstetrics and Gynecology, The affiliated Hospital of Guizhou Medical University, Guiyang, China.
Autoimmunity. 2021 May;54(3):156-162. doi: 10.1080/08916934.2021.1907572. Epub 2021 Apr 1.
Indoleamine 2,3-dioxygenase1(IDO1) is one of the most important proteins in protect the embryos from the mother's immune system during pregnancy. However, the regulation of the protein expression at the maternal-foetal interface is not fully known. We aimed to study the regulation of IDO1 expression by progesterone in villi and decidua of in early pregnancy. Fifty cases of early pregnancy women's villi and decidua were collected. Tissue explants of chorionic villi and the decidua were cultured in media containing in different concentrations of progesterone, in the presence or absence of mifepristone. Western blot analysis and immunofluorescence were used to detect the expression of IDO1 in chorionic villi and decidua in cultured tissues. IDO1 protein was identified in chorionic villi and decidua tissues of normal pregnant women, and the expression of IDO1in the decidua was significantly higher than those in chorionic villi. Progesterone decreased IDO1 expression in early pregnancy chorionic villi and decidua, and mifepristone, as the progesterone inhibitor, reverted this effect. In normal physiological state of pregnancy, progesterone may be involved in the regulation of immune tolerance by negative regulation of IDO1 expression at maternal foetal interface. Progesterone may down-regulate IDO1 expression during early pregnancy.
吲哚胺2,3-双加氧酶1（IDO1）是孕期保护胚胎免受母体免疫系统攻击的最重要蛋白质之一。然而，母胎界面处该蛋白质表达的调控机制尚不完全清楚。我们旨在研究孕早期绒毛和蜕膜中孕酮对IDO1表达的调控作用。收集了50例早孕妇女的绒毛和蜕膜。将绒毛膜绒毛和蜕膜的组织外植体在含有不同浓度孕酮的培养基中培养，同时添加或不添加米非司酮。采用蛋白质免疫印迹分析和免疫荧光法检测培养组织中绒毛膜绒毛和蜕膜中IDO1的表达。在正常孕妇的绒毛膜绒毛和蜕膜组织中鉴定出IDO1蛋白，且IDO1在蜕膜中的表达显著高于绒毛膜绒毛。孕酮降低了孕早期绒毛膜绒毛和蜕膜中IDO1的表达，而作为孕酮抑制剂的米非司酮则逆转了这种作用。在正常妊娠生理状态下，孕酮可能通过对母胎界面处IDO1表达的负调控参与免疫耐受的调节。孕酮可能在孕早期下调IDO1的表达。
