Liu Wei, Huang Yongli, Huang Guanyou, Zhou Congrong, Zeng Xiaoling, Zhao Shuyun, Wu Lingfei, Zhou Hua, Wu Qingqing, Dai Lujun
Reproductive Medicine Center, Department of Obstetrics and Gynecology, Affiliated Hospital of Guizhou Medical University, Guiyang, Guizhou 550004, P.R. China.
Reproductive Center, People's Hospital of Guizhou Province, Guiyang, Guizhou 550003, P.R. China.
Exp Ther Med. 2017 Nov;14(5):4817-4824. doi: 10.3892/etm.2017.5142. Epub 2017 Sep 19.
We aimed to investigate the expression of suppressors cytokine signaling (SOCS)-3, transforming growth factor (TGF)-β and indoleamine 2,3-dioxygense (IDO) and to analyse the relationship of SOCS3 and TGF-β with IDO expression in early pregnancy chorionic villi and decidua in the maternal-fetal interface. Western blot analysis and immunohistochemical method were used to detect the expression of TGF-β, SOCS3 and IDO in chorionic villi and decidua tissues of normal pregnant women. SOCS3, TGF-β and IDO protein was identified in chorionic villi and decidua tissues of normal pregnant women and there was a negative correlation between the expression of IDO and SOCS3, but TGF-β expression was positively correlated with IDO expression. The levels of IDO expression in the decidua from normal pregnancies were significantly higher than those in chorionic villi, while the expression of SOCS3 was no significant difference between decidua and chorionic villi. In normal physiological state of pregnancy, SOCS3 and TGF-β may be involved in the regulation of immune tolerance by positive or negative regulation of IDO expression at maternal fetal interface.
我们旨在研究细胞因子信号转导抑制因子（SOCS）-3、转化生长因子（TGF）-β和吲哚胺2,3-双加氧酶（IDO）的表达，并分析母胎界面早期妊娠绒毛膜绒毛和蜕膜中SOCS3和TGF-β与IDO表达的关系。采用蛋白质免疫印迹分析和免疫组织化学方法检测正常孕妇绒毛膜绒毛和蜕膜组织中TGF-β、SOCS3和IDO的表达。在正常孕妇的绒毛膜绒毛和蜕膜组织中鉴定出SOCS3、TGF-β和IDO蛋白，IDO与SOCS3的表达呈负相关，但TGF-β表达与IDO表达呈正相关。正常妊娠蜕膜中IDO表达水平显著高于绒毛膜绒毛，而SOCS3在蜕膜和绒毛膜绒毛中的表达无显著差异。在正常妊娠生理状态下，SOCS3和TGF-β可能通过对母胎界面IDO表达的正负调节参与免疫耐受的调控。
