Reich D J, Keller S E
Department of Psychiatry, Mount Sinai School of Medicine, NY 10029.
J Immunol Methods. 1988 Jun 13;110(2):179-81. doi: 10.1016/0022-1759(88)90101-9.
A method of obtaining rat thoracic duct lymphocytes is described, including a review of the surgical technique, with several modifications, and including an outline of a newly designed rat restrainer that helps to triple thoracic duct output compared to the Bollman restrainer. The new restrainer allows the rat increased mobility but protects the thoracic duct cannula. Unanesthetized 200 g rats yielded 80 ml/day thoracic duct lymph containing 4.2 x 10(4) lymphocytes/ml.
本文描述了一种获取大鼠胸导管淋巴细胞的方法，包括对手术技术的回顾，其中有若干改进，还包括一种新设计的大鼠固定器的概述，与博尔曼固定器相比，该固定器有助于使胸导管输出量增加两倍。这种新固定器能让大鼠有更大的活动自由度，但能保护胸导管插管。未麻醉的200克大鼠每天可产生80毫升胸导管淋巴液，每毫升含有4.2×10⁴个淋巴细胞。
